Essential oils extracted from plants provide an efficient and eco-friendly alternate to manage the challenge of mosquito-borne diseases. The use of these oils as larvicidal agents, however, poses the problem of their improper dispersion in larval aquatic habitats. To overcome this, the methodology for the synthesis of Eucalyptus globulus oil nanoemulsions in water was standardised by mixing the oil in water with Tween^®-20 in three different ratios (1:1, 1:2 and 1:3) by sonication. On the basis of thermodynamic stability tests, oil emulsions having E. globulus oil and Tween^®-20 in 1:2 ratio was found to be the most stable which was characterised using transmission electron microscopy (TEM). The droplets of prepared nanoemulsions were found to have an average size of 40 nm and were spherical in shape. The larvicidal activity of stable aqua nanoemulsions of eucalyptus oil was tested against Aedes aegyptiusing five different concentrations at 100, 90, 80, 70, 60 and 50 ppm (in triplicate). LC₅₀ and LC₉₀ values were found to be 60.30 and 92.20 ppm. Observations revealed that 70 ppm was the most effective concentration, causing 100 % mortality within 24 h.