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1 April 2004 Interactions of Butyric Acid– and Acetic Acid–Treated Salmonella with Chicken Primary Cecal Epithelial Cells In Vitro
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Abstract

In vitro studies of the interaction between pathogenic bacteria and the chicken intestinal epithelium are hampered by the lack of a host- and tissue-specific in vitro model. Therefore, a reproducible method for isolation and cultivation of chicken primary cecal epithelial cells was developed. Cecal crypts were isolated and cultured in vitro to form a semiconfluent layer of epithelial cells. Incubation of Salmonella enteritidis with these cells resulted in invasion. Pretreatment of the Salmonella bacteria with butyric acid resulted in a significant decrease of invasion of the bacteria in the chicken cecal epithelial cells, whereas pretreatment with acetic acid increased invasiveness. These interactions of S. enteritidis with primary chicken cecal epithelial cells were similar to the interactions with other epithelial cell types.

Filip Van Immerseel, Jeroen De Buck, Isabel De Smet, Frank Pasmans, Freddy Haesebrouck, and Richard Ducatelle "Interactions of Butyric Acid– and Acetic Acid–Treated Salmonella with Chicken Primary Cecal Epithelial Cells In Vitro," Avian Diseases 48(2), 384-391, (1 April 2004). https://doi.org/10.1637/7094
Received: 5 August 2003; Published: 1 April 2004
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