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1 June 2005 Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus
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Abstract

A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian encephalomyelitis virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural protein P1 region of the AEV genome. An avian encephalomyelitis virus-specific 619-base pair cDNA product was amplified by these primers from five reference/field strains of AEVs but not from 10 other avian pathogenic viruses and bacteria. The RT-PCR assay developed in this study was found to be sensitive and specific with as little as 10 pg of avian encephalomyelitis virus RNA detected using gel electrophoresis. Furthermore, AEV-RT-PCR was able to detect AE virus from chicken embryo brain at 3 days postinoculation as compared with the AE agar gel precipitation test (AGP), which required up to 11 days of incubation in the embryos.

Zhiqin Xie, Mazhar I. Khan, Theodore Girshick, and Zhixun Xie "Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus," Avian Diseases 49(2), 227-230, (1 June 2005). https://doi.org/10.1637/7307-111804R
Received: 18 November 2004; Accepted: 1 January 2004; Published: 1 June 2005
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