Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
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1 December 2005
Cecal Spirochetosis Caused by Brachyspira pilosicoli in Commercial Turkeys
H. L. Shivaprasad,
G. E. Duhamel
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Avian Diseases
Vol. 49 • No. 4
December 2005
Vol. 49 • No. 4
December 2005
bp = base pair
Brachyspira pilosicoli
ceca
FFPE = formalin-fixed and paraffin-embedded
H&E = hematoxylin and eosin
HEV = hemorrhagic enteritis virus
HI = hemagglutination inhibition