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1 December 2005 An Allele-Specific PCR Assay for the Rapid and Serotype-Specific Detection of Salmonella Pullorum
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Salmonella serovar Pullorum is a causative agent of pullorum disease (PD) in poultry and is responsible for severe economic losses to the poultry industry in many parts of the world. A definitive detection of Pullorum requires culture followed by serotyping and biochemical identification, a process that is tedious and takes several weeks to accomplish. We have developed a rapid allele-specific polymerase chain reaction (PCR) method based on the nucleotide polymorphism in rfbS gene sequence for the serotype-specific detection of Pullorum and its differentiation from the closely related Gallinarum. The specificity of this PCR assay was tested using DNA samples from Pullorum (n = 13), Salmonella serotypes other than Pullorum (n = 19), and closely related non-Salmonella organisms (n = 5). The PCR assay was highly serotype-specific as the PCR amplicon of 147 base pairs was observed only in the case of Pullorum, while all the other DNA samples tested PCR negative. A definitive identification of Pullorum cultures was possible in less than 3 hr. As little as 100 pg of SP DNA was detected. This allele-specific PCR method is highly specific as well as sensitive and may be an effective molecular tool in the rapid and serotype-specific detection of Pullorum and differentiation from other Salmonella species.

Atul R. Desai, Devendra H. Shah, Smriti Shringi, Mi-Jin Lee, Ying-Hua Li, Mae-Rim Cho, Jin-Ho Park, Seong-Kug Eo, John-Hwa Lee, and Joon-Seok Chae "An Allele-Specific PCR Assay for the Rapid and Serotype-Specific Detection of Salmonella Pullorum," Avian Diseases 49(4), 558-561, (1 December 2005).
Received: 22 May 2005; Accepted: 1 August 2005; Published: 1 December 2005

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