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20 November 2012 Isolation and Genotyping of Toxoplasma gondii from Free-Range Ducks in Malaysia
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Abstract

Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of humans. The present study was performed to isolate and genotype T. gondii from free-range ducks in Malaysia. Sera, heads, and hearts from 205 ducks were obtained from four states in Peninsular Malaysia, and 30 (14.63%) sera were found to be seropositive when assayed with the modified agglutination test (MAT ≥ 1∶6). All the positive samples were inoculated into mice, and T. gondii was successfully isolated from four individual duck samples (1.95%), which were initially found to be strongly seropositive (MAT ≥ 1∶24). The isolates were subjected to PCR-RFLP analysis, and two T. gondii strains were identified: type I and type II. This is the first reported study on the genetic characterization of T. gondii isolates from free-range farm animals in Southeast Asia.

Nota de Investigación—Aislamiento y genotipificación de Toxoplasma gondii de patos criados en semi-pastoreo en Malasia.

Toxoplasma gondii es un parásito protozoario que infecta a casi un tercio de los seres humanos. El presente estudio se realizó para aislar y genotipificar T. gondii de patos criados en semi-pastoreo en Malasia. Se obtuvieron muestras de suero, cabezas y corazones de 205 patos de cuatro estados de Malasia Peninsular. Treinta sueros (14.63%) resultaron positivos cuando se analizaron mediante una prueba de aglutinación modificada (MAT ≥ 1∶6). Todas las muestras positivas fueron inoculadas en ratones, y T. gondii se aisló con éxito a partir de cuatro muestras individuales de patos (1.95%), los cuales resultaron ser fuertemente seropositivos (MAT ≥ 1∶24) inicialmente. Los aislados fueron sometidos al análisis por PCR-RFLP y se identificaron dos cepas de T. gondii: tipo I y tipo II. Este es el primer estudio publicado sobre la caracterización genética de cepas de T. gondii a partir de animales mantenidos en semi-pastoreo en el sudeste de Asia.

American Association of Avian Pathologists
Vignesh R. Puvanesuaran, Rahmah Noordin, and Venugopal Balakrishnan "Isolation and Genotyping of Toxoplasma gondii from Free-Range Ducks in Malaysia," Avian Diseases 57(1), 128-132, (20 November 2012). https://doi.org/10.1637/10304-071212-ResNote.1
Received: 14 July 2012; Accepted: 1 November 2012; Published: 20 November 2012
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