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23 February 2015 Isolation, Identification, and Sequencing of a Goose-Derived Newcastle Disease Virus and Determination of Its Pathogenicity
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Abstract

In August 2010, geese in the Meihekou area of Jilin province in China were found to be infected by a pathogen that caused a disease similar to Newcastle disease. To determine the causative agent of the infections, a virus was isolated from liver tissues of infected geese, followed by a pathogenicity determination. The isolated virus was named NDV/White Goose/China/Jilin(Meihekou)/MHK-1/2010. Specific primers were designed to amplify the whole genome of the MHK-1 virus, followed by sequencing and splicing of the entire genome. Sequencing and phylogenetic analysis of MHK-1 showed that the isolate was a virulent strain of Newcastle disease virus. The MHK-1 genome is 15,192 nucleotides long, and it belongs to the class II branch of Newcastle disease viruses, as evidenced by the amino acid sequence (112R-R-Q-K-R-F117) of the F protein. The hemagglutinin titer was 1:128 to 1:512. The chicken embryo mean death time, the intracerebral pathogenicity index, and the median lethal dose of chicken embryos of MHK-1 were 43 hr, 1.63, and 109/ml, respectively, which revealed that the newly isolated MHK-1 strain is strongly pathogenic to geese.

© 2015 American Association of Avian Pathologists
Xiao-Qing Chen, Zi-Bing Li, Gui-Xue Hu, Song-Zhi Gu, Shuang Zhang, Ying Ying, and Feng-Shan Gao "Isolation, Identification, and Sequencing of a Goose-Derived Newcastle Disease Virus and Determination of Its Pathogenicity," Avian Diseases 59(2), 235-243, (23 February 2015). https://doi.org/10.1637/10957-100914-Reg
Received: 13 October 2014; Accepted: 1 February 2015; Published: 23 February 2015
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