The queen conch, Strombus gigas, is a highly valued fishery resource in the Caribbean basin, historically second only to the spiny lobster. Currently listed in Appendix II of CITES, historical surveys from the U.S. Virgin Islands have documented wide-spread reductions in population densities. Habitat use and movement patterns of queen conch differ by size and maturity status, making it important to identify areas and habitat types that can play a role in the recovery of this species. We tracked long-term movements and spatial distributions of conch in St. John, USVI, using visual surveys coupled with mark-and-recapture techniques. Juvenile conch exhibited the smallest mean home range (95% contour) and core area (50% contour) at 8470 m² and 2083 m², respectively, but had the largest aggregate home range (115,410 m²) and aggregate core area (55,055 m²). Transitional conch had the largest mean home range and core area at 18,203 m² and 4944 m², respectively, with an aggregate home range and core area of 113,147 m² and 59,067 m². Adults had an aggregate home range and core area of 65,045 m² and 29,178 m², with a mean home range and core area of 14,987 m² and 3929 m². Adults exhibited the highest daily movement rate at 11.36 m per day, with juvenile and transitional conch having similar rates of 4.66 m per day and 3.44 m per day, respectively. Multiple recapture events of tagged conch showed an ontogenetic shift in habitat use as maturing individuals moved from shallow seagrass habitats into deeper-water macroalgae plains.