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1 June 2014 Effect of Holding Diet on Egg Formation of Tamarixia radiata (Hymenoptera: Eulophidae), Parasitoid of Diaphorina citri (Hemiptera: Psylloidae)
Xulin Chen, Philip A. Stansly
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Abstract

Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae), is an arrhenotokous ectoparasitoid of Diaphorina citri Kuwayama (Hemiptera: Psylloidae), vector of huanglongbing (HLB) or citrus greening disease. Mass-rearing parasitoids for augmentative biological control has created the need to find storage conditions that will optimize egg load upon release. Food provided to females during the holding period may be foremost among factors that determine the number of eggs available for oviposition following storage. Pairs of newly emerged T. radiata were provided with 8 different diet treatments: water, honey, Nu-Lure® (a proteinaceous liquid made from 44% hydrolyzed corn gluten meal), host nymphs, honey Nu-Lure, honey host nymphs, Nu-Lure host nymphs, and honey Nu-Lure host nymphs. Female wasps were dissected after 5, 10, 15, and 20 days and mature eggs counted. An average of 4.6 eggs was observed in ovaries of newly emerged females. Honey alone was sufficient for female survival, but egg resorption took place within 5 days after emergence. The combination of honey Nu-Lure resulted in female survivorship similar to a diet of host nymphs, but egg formation was less than with nymphs provided. Tamarixia radiata formed more eggs feeding on mixed diets (Nu-Lure honey nymphs or Nu-Lure nymphs) compared to nymphs alone. However no artificial diet substituted for nymphal hemolymph.

Xulin Chen and Philip A. Stansly "Effect of Holding Diet on Egg Formation of Tamarixia radiata (Hymenoptera: Eulophidae), Parasitoid of Diaphorina citri (Hemiptera: Psylloidae)," Florida Entomologist 97(2), 491-495, (1 June 2014). https://doi.org/10.1653/024.097.0220
Published: 1 June 2014
KEYWORDS
almacenamiento
biological control
Control biológico
cría masiva
diet
Dieta
mass-rearing
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