Trypetidomima Townsend is a Neotropical Rhinophoridae genus with a single species, T. lutea Townsend, from southeastern Brazil. Based on comprehensive material from Atlantic Forest localities, we found a new species of Trypetidomima, herein described and named as T. fusca sp. nov., with geographical occurrence in the State of São Paulo, southeastern Brazil. With the addition of this new species, the genus is redefined, and an identification key is provided to distinguish between the 2 known species. The type-species, T. lutea Townsend, is redescribed, including the description of the previously unknown female.