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1 June 2012 Intron Structure of the Elongation Factor 1-Alpha Gene in the Ponto-Caspian Amphipod Pontogammarus maeoticus (Sowinsky, 1894) and Its Phylogeographic Utility
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Abstract

We tested the utility of a 230 base pair intron fragment of the highly conserved nuclear gene Elongation Factor 1-alpha (EF1-α) as a proper marker to reconstruct the phylogeography of the marine amphipod Pontogammarus maeoticus (Sowinsky, 1894) from the Caspian and Black Seas. As a prerequisite for further analysis, we confirmed by Southern blot analysis that EF1-α is encoded at a single locus in P. maeoticus. We included 15 populations and 60 individuals in the study. Both the phylogeny of the 27 unique alleles found and population genetic analyses revealed a significant differentiation between populations from the aforementioned sea basins. Our results are in remarkable agreement with recent studies on a variety of species from the same area, which invariably support a major phylogeographic break between the Caspian and Black Seas. We thus conclude that our EF1-α intron is an informative marker for phylogeographic studies in amphipods at the shallow population level.

© The Crustacean Society, 2012.
Nahid Nahavandi, Valerio Ketmaier, and Ralph Tiedemann "Intron Structure of the Elongation Factor 1-Alpha Gene in the Ponto-Caspian Amphipod Pontogammarus maeoticus (Sowinsky, 1894) and Its Phylogeographic Utility," Journal of Crustacean Biology 32(3), 425-433, (1 June 2012). https://doi.org/10.1163/193724012X626584
Received: 20 May 2011; Accepted: 1 November 2011; Published: 1 June 2012
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