Soil microbes play important roles in terrestrial ecosystem carbon and nitrogen cycling. Climatic warming and elevated CO₂ are two aspects of climatic change. In this study, we used a meta-analysis approach to synthesise observations related to the effects of warming and elevated CO₂ on soil microbial biomass and community structure. Ecosystem types were mainly grouped into forests and grasslands. Warming methods included open top chambers and infrared radiators. Experimental settings included all-day warming, daytime warming and nighttime warming. Warming increased soil actinomycetes and saprotrophic fungi, while elevated CO₂ decreased soil gram-positive bacteria (G⁺). Mean annual temperature and mean annual precipitation were negatively correlated with warming effects on gram-negative bacteria (G^–) and total phospholipid fatty acid (PLFA), respectively. Elevation was positively correlated with the warming effect on total PLFA, bacteria, G⁺ and G^–. Grassland exhibited a positive response of total PLFA and actinomycetes to warming, while forest exhibited a positive response in the ratio of soil fungi to bacteria (F/B ratio) to warming. The open top chamber method increased G^–, while the infrared radiator method decreased the F/B ratio. Daytime warming rather than all-day warming increased G^–. Our findings indicated that the effects of warming on soil microbes differed with ecosystem types, warming methods, warming times, elevation and local climate conditions.