This study identified the most suitable fluorochrome to mark prodissoeonch of larval Siphonaria australis, a common pulmonate gastropod commonly found on rocky shores throughout New Zealand. The pulmonate habit of laying attached larval egg masses makes them an excellent candidate for studies of larval connectivity using chemical staining methods. Larval S. australis egg masses were immersed in 200 mg/L of either alizarin red S (ARS), calcein, or Oxytetracycline (OTC) for 24 h before viewing under ultraviolet light to allow quantification of mark brightness using image analysis software. Counts of pre- and postimmersion mortality were also made to identify any fluorochrome toxicity to S. australis larvae. Calcein was found to be the most suitable fluorochrome to use when fluorescently marking larval S. australis, because average brightness was significantly greater than either ARS or OTC, with negligible mortality levels recorded for calcein. The results show that fluorochromes can penetrate the gelatinous egg matrix and the larval capsules, thus providing an opportunity for staining large numbers of these species for alongshore connectivity studies.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 29 • No. 4