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1 September 2014 Identification of Indian Mangrove Mud Crab Genus Scylla spp. (Brachyura: Portunidae) using RAPD and PCR-RFLP Markers
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Abstract

Identification of commonly available Indian mud crabs of the genus Scylla was carried out using RAPD and PCR-RFLP to resolve the taxonomic ambiguity of the genus. Samples were collected from western, eastern, and Andaman coastal waters of India. A total of 16 potential species-specific RAPD markers were identified from 179 individuals using 4 arbitrary primers (OPA2, OPA14, UBC122, and UBC456). Three PCR-RFLP markers were developed that could be used to confirm taxonomic status of Indian mud crab species. The species-specific RAPD markers and the digestion pattern of PCR-RFLP analysis indicate conclusively that only 2 species of mud crabs (Scylla serrata and Scylla olivacea) are commonly present in Indian coastal waters.

Anup Mandal, Mathews Varkey, Anjali Kottayil Mani, Sobha Pindaniyil Sobhanan, and Yohannan Chellamma Thampi-Samraj "Identification of Indian Mangrove Mud Crab Genus Scylla spp. (Brachyura: Portunidae) using RAPD and PCR-RFLP Markers," Journal of Shellfish Research 33(2), 503-510, (1 September 2014). https://doi.org/10.2983/035.033.0219
Published: 1 September 2014
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