Prior to 1998 no record of a Seminole bat (Lasiurus seminolus) had been recorded in Kentucky, despite extralimital records of this species occurring in several states farther north in latitude. As of 2014, 12 specimens of the species have been recorded in Kentucky, with Seminole bats captured or recovered in Barren, Edmonson, Fayette, Lyon, and Trigg counties; an expanse of the state exceeding 300 km. Records include both juvenile and adult bats of males and females, with two reproductively active females captured in Trigg County in 2002 and 2005. Seminole bats were captured as early as 5 July and as late as 17 September, with 66.7 % of specimens recorded during the summer maternity season. The relatively recent preponderance of Seminole bat records across Kentucky suggests these bats are infrequent visitors to the state, at least during the mid-summer to autumn seasons, and are potentially breeding in the southwestern region near Land Between the Lakes National Recreation Area. We hypothesize that continued range expansion of the Seminole bat could result in this species becoming a breeding-summer resident in Kentucky, likely inhabiting yellow-pine forests in the southernmost counties of the state.