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1 August 2000 Effect of Solvent–Water Mixtures on the Prototropic Equilibria of Fluorescein and on the Spectral Properties of the Monoanion
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Abstract

A spectral resolution procedure was used to resolve the absorption, excitation and emission spectra of the fluorescein monoanion in a number of solvent–water mixtures. This permitted an analysis of the effect of the solvent environment on the spectral properties of the monoanion and on the lactone/monoanion/dianion transitions of fluorescein. The monoanion excitation and emission spectra show relatively small changes with changing environment, a behavior that is related to the hydrogen-bonding environment of the solvent–water mixtures. There is also a general increase in the quantum yield of the monoanion from 0.36 in water to values up to 0.49 in the solvent–water mixtures. The presence of solvent also results in a general increase in the lactone content and in the monoanion:dianion and lactone:monoanion ratios. General polarity effects alone cannot account for the observed effects on the prototropic transitions indicating that specific solute–solvent effects involving hydrogen bonding perturb the prototropic equilibria of fluorescein.

Nectarios Klonis and William H. Sawyer "Effect of Solvent–Water Mixtures on the Prototropic Equilibria of Fluorescein and on the Spectral Properties of the Monoanion," Photochemistry and Photobiology 72(2), 179-185, (1 August 2000). https://doi.org/10.1562/0031-8655(2000)072<0179:EOSWMO>2.0.CO;2
Received: 18 January 2000; Accepted: 1 May 2000; Published: 1 August 2000
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