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1 September 2000 Exposure to UVB Induces Accumulation of LFA-1 T Cells and Enhanced Expression of the Chemokine Psoriasin in Normal Human Skin
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Abstract

Normal human skin shows preferential (epi)dermal infiltration of CD4 T cells upon acute UV exposure. To study the mechanism behind this feature we locally exposed healthy volunteers to doses of UV commonly encountered by the population. Expression of integrins on T cells and expression of adhesion molecules on dermal endothelial cells were quantitatively assessed by immunohistochemistry in situ. We also investigated the effects of ultraviolet-B (UVB) exposure on psoriasin and IL-16, two specific chemoattractant factors for CD4 T cells, at messenger RNA (mRNA) level by semiquantitative reverse transcriptase-polymerase chain reaction and at protein level by immunohistochemistry. We found, at day 2 after exposure to four minimal erythema doses of UVB, predominant accumulation of LFA-1 /CLA/VLA-4 T cells in the dermis. Concomitantly the expression of ICAM-1, but not that of E-selectin and VCAM-1, was upregulated on dermal endothelial cells. The increase in the number of dermal T cells was not due to proliferation because only 2% of the UVB-induced dermal T cells expressed the marker of proliferation Ki-67. Whereas exposure to 35 J/cm2 of ultraviolet-A (UVA), like UVB, induced a loss of intraepidermal T cells at day 2 after exposure, UVA induced neither any influx of T cells into the dermis nor any adhesion molecule upregulation on endothelial cells. In response to UVB exposure, the expression of psoriasin mRNA, but not of IL-16 mRNA, was upregulated; the expression of psoriasin protein was also found to be upregulated. These results suggest that LFA-1/ICAM-1 pathway and psoriasin are both involved in the accumulation of CD4 T cells into UVB-irradiated skin, possibly via a recruitment mechanism.

Sergio Di Nuzzo, Regien M. R. Sylva-Steenland, Cornelis W. Koomen, Menno A. de Rie, Pranab K. Das, Jan D. Bos, and Marcel B. M. Teunissen "Exposure to UVB Induces Accumulation of LFA-1 T Cells and Enhanced Expression of the Chemokine Psoriasin in Normal Human Skin," Photochemistry and Photobiology 72(3), 374-382, (1 September 2000). https://doi.org/10.1562/0031-8655(2000)072<0374:ETUIAO>2.0.CO;2
Received: 26 January 2000; Accepted: 1 June 2000; Published: 1 September 2000
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