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1 October 2000 Singlet Oxygen, but not Oxidizing Radicals, Induces Apoptosis in HL-60 Cells
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Abstract

Oxidizing species (OS), produced by photosensitization or derived from cytotoxic agents, activate apoptotic pathways. We investigated whether two different OS, formed at the same subcellular sites, have equivalent ability to initiate apoptosis in HL-60 cells. Our previous work showed that absorption of visible light by rose bengal (RB) produces singlet oxygen exclusively, whereas absorption of ultraviolet A produces RB-derived radicals in addition to singlet oxygen. Singlet oxygen, but not the RB-derived radicals, induced nuclear condensation and DNA fragmentation into nucleosome-size fragments in a dose dependent manner. In contrast, the RB-derived radicals caused greater lipid oxidation than singlet oxygen. These results indicate that different OS, produced at the same subcellular sites, do not have the same ability to induce apoptosis and that the ability of an OS to initiate lipid oxidation does not necessarily correlate with its ability to induce apoptosis.

Irene E. Kochevar, Mary C. Lynch, Shougang Zhuang, and Christopher R. Lambert "Singlet Oxygen, but not Oxidizing Radicals, Induces Apoptosis in HL-60 Cells," Photochemistry and Photobiology 72(4), 548-553, (1 October 2000). https://doi.org/10.1562/0031-8655(2000)072<0548:SOBNOR>2.0.CO;2
Received: 18 April 2000; Accepted: 1 July 2000; Published: 1 October 2000
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