Translator Disclaimer
1 June 2001 In Vitro Induction of PDT Resistance in HT29, HT1376 and SK-N-MC Cells by Various Photosensitizers
Author Affiliations +
Abstract

Our approach to examine the mechanism(s) of action for photodynamic therapy (PDT) has been via the generation of PDT-resistant cell lines. In this study we used three human cell lines, namely, human colon adenocarcinoma (HT29), human bladder carcinoma and human neuroblastoma. The three photosensitizers used were Photofrin, Nile Blue A and aluminum phthalocyanine tetrasulfonate. The protocol for inducing resistance consisted of repeated in vitro photodynamic treatments with a photosensitizer to the 1–10%-survival level followed by regrowth of single surviving colonies. Varying degrees of resistance were observed. The three induced variants of the HT29 cell line were the most extensively studied. Their ratios of increased survival at the LD90 level range between 1.5- and 2.62-fold more resistant.

Gurmit Singh, Myrna Espiritu, Xiao Yun Shen, John G. Hanlon, and Andrew J. Rainbow "In Vitro Induction of PDT Resistance in HT29, HT1376 and SK-N-MC Cells by Various Photosensitizers," Photochemistry and Photobiology 73(6), 651-656, (1 June 2001). https://doi.org/10.1562/0031-8655(2001)073<0651:IVIOPR>2.0.CO;2
Received: 18 September 2000; Accepted: 1 March 2001; Published: 1 June 2001
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top