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1 September 2004 Comparative Sensitivity of Microvascular Endothelial Cells, Fibroblasts and Tumor Cells after In Vitro Photodynamic Therapy with meso-Tetra-Hydroxyphenyl-Chlorin
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Abstract

The phototoxic effect of meso-tetra-hydroxyphenyl-chlorin (mTHPC)–mediated photodynamic therapy (PDT) on human microvascular endothelial cells (hMVEC) was compared with that on human fibroblasts (BCT-27) and two human tumor cell lines (HMESO-1 and HNXOE). To examine the relationship between intrinsic phototoxicity and intracellular mTHPC content, we expressed cell survival as a function of cellular fluorescence. On the basis of total cell fluorescence, HNXOE tumor cells were the most sensitive and BCT-27 fibroblasts the most resistant, but these differences disappeared after correcting for cell volume. Endothelial cells were not intrinsically more sensitive to mTHPC-PDT than tumor cells or fibroblasts. Uptake of mTHPC in hMVEC increased linearly to at least 48 h, whereas drug uptake in the other cell lines reached a maximum by 24 h. No difference in drug uptake was seen between the cell lines during the first 24 h, but by 48 h hMVEC had a 1.8- to 2.8-fold higher uptake than other cell lines. Endothelial cells showed a rapid apoptotic response after mTHPC-mediated PDT, whereas similar protocols gave a delayed apoptotic or necrotic like response in HNXOE. We conclude that endothelial cells are not intrinsically more sensitive than other cell types to mTHPC-mediated PDT but that continued drug uptake beyond 24 h may lead to higher intracellular drug levels and increased photosensitivity under certain conditions.

Martijn Triesscheijn, Marjan Ruevekamp, Maurice Aalders, Paul Baas, and Fiona A. Stewart "Comparative Sensitivity of Microvascular Endothelial Cells, Fibroblasts and Tumor Cells after In Vitro Photodynamic Therapy with meso-Tetra-Hydroxyphenyl-Chlorin," Photochemistry and Photobiology 80(2), 236-241, (1 September 2004). https://doi.org/10.1562/2004-03-19-RA-120.1
Received: 19 March 2004; Accepted: 1 June 2004; Published: 1 September 2004
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