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1 December 2004 Photochemical Reduction of Cytochrome c by a 1,4,5,8-Naphthalenediimide Radical Anion
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Abstract

Steady-state UV irradiation of aqueous solutions containing cytochrome c (cyt c) and N,N′-bis(2-phosphonoethyl)-1,4,5,8-naphthalenediimide (BPNDI), a water-soluble aromatic imide, resulted in the reduction of the heme iron from the Fe(III) to the Fe(II) oxidation state. The reaction kinetics were followed by the increase of the ferrocytochrome c absorbance band at 549 nm. The rate of the photochemical reaction was pH dependent, reaching its maximum values over the pH range 4–7. Addition of electrolyte (NaCl) at pH 5 resulted in a decrease in the reaction rate, as expected for reactions between oppositely charged species. Flash photolysis studies revealed that the actual reductant in the reaction was a photogenerated BPNDI radical anion, which transferred an electron to the cyt c heme iron. The participation of imide radicals in the process was confirmed by the ready reduction of cyt c by BPNDI radicals chemically generated with sodium dithionite.

Ivana B. Campos, Iseli L. Nantes, Mario J. Politi, and Sergio Brochsztain "Photochemical Reduction of Cytochrome c by a 1,4,5,8-Naphthalenediimide Radical Anion," Photochemistry and Photobiology 80(3), 518-524, (1 December 2004). https://doi.org/10.1562/0031-8655(2004)080<0518:PROCCB>2.0.CO;2
Received: 1 July 2004; Accepted: 1 August 2004; Published: 1 December 2004
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