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1 March 2005 Two-photon Excitation Induced Fluorescence of a Trifluorophore-labeled DNA
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Abstract

Two-photon excitation of a trifluorophore (6-carboxyfluorescein, N,N,N′,N′-tetramethyl-6-carboxyrhodamine and cyanine-5 monofunctional dye) labeled DNA, which has a scaffold of 26 nucleotides, was achieved using focused laser light of a Q-switched Nd-YAG laser (1064 nm). The observed fluorescence signature (emission ratio from the three fluorophores) of the labeled DNA after two-photon excitation is very different from the fluorescence signatures produced by one-photon excitation at different wavelength. The additional fluorescence signatures produced by two-photon excitation of the fluorescent oligonucleotides will facilitate their use as combinatorial fluorescence energy transfer tags for multiplex genetic analysis.

Steffen Jockusch, Zengmin Li, Jingyue Ju, and Nicholas J. Turro "Two-photon Excitation Induced Fluorescence of a Trifluorophore-labeled DNA," Photochemistry and Photobiology 81(2), 238-241, (1 March 2005). https://doi.org/10.1562/2004-09-27-RA-329.1
Received: 27 September 2004; Accepted: 1 January 2005; Published: 1 March 2005
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