Translator Disclaimer
1 July 2005 Monitoring Cell Survival After Extraction of a Single Subcellular Organelle Using Optical Trapping and Pulsed-Nitrogen Laser Ablation
Author Affiliations +
Abstract

This paper characterizes cell viability in three different cell lines—Chinese hamster ovary cells (CHO), neuroblastoma cells fused with glialoma cells (NG108-15) and murine embryonic stem cells (ES-D3)—after N2 laser disruption of the cell membrane and removal, via optical trapping, of a single subcellular organelle. Morphological changes and viability (as determined by live/dead fluorescent stains) of the cell were monitored every half hour over a 4-h period postsurgery. The ability of the cell to survive organelle extraction was found to depend both on the conditions under which surgery was performed and on the cell type. The average viability after surgery for CHO cells was approximately 80%, for NG 108 cells it was approximately 30% and for ES-D3 cells postsurgery viability was approximately 10%. From over 600 surgeries we found the survival of the cell is determined almost exclusively within the first hour postsurgery regardless of cell line. The optimal pulse energy for N2 laser ablation was approximately 0.7 μJ. The N2 pulse produced an approximately 1–3 μm hole in the cell membrane and proved to be the primary source of cell death in those cells that did not survive the procedure.

J. Patrick Shelby, J. Scott Edgar, and Daniel T. Chiu "Monitoring Cell Survival After Extraction of a Single Subcellular Organelle Using Optical Trapping and Pulsed-Nitrogen Laser Ablation," Photochemistry and Photobiology 81(4), 994-1001, (1 July 2005). https://doi.org/10.1562/2005-02-02-RA-431R1.1
Received: 2 February 2005; Accepted: 1 April 2005; Published: 1 July 2005
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top