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1 November 2005 Circular Dichroism of the Photoreceptor Pigment Oxyblepharismin
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Abstract

Circular dichroism (CD) was used to study the structure of oxyblepharismin (OxyBP), the photoreceptor chromophore for the photophobic response of the blue form of Blepharisma japonicum. Both the chromophore associated to its native protein and the free chromophore in ethanol solution were investigated. CD spectra in the far-UV range indicate that OxyBP induces a slight increase in the α-helix content of the protein matrix. CD spectra in the near-UV and visible region of the spectrum show that OxyBP adopts a chiral conformation with a preferential geometry not only when associated to its protein matrix, but also when isolated and dissolved in ethanol. This experimental result is related to the existence of a high-energy interconversion barrier between two enantiomeric structures of the molecule and discussed on the basis of an asymmetric biosynthesis of its precursor, blepharismin.

Osvaldo Pieroni, Pascal Plaza, Mathilde Mahet, Nicola Angelini, Giovanni Checcucci, Manuela Malatesta, Monique M. Martin, and Francesco Lenci "Circular Dichroism of the Photoreceptor Pigment Oxyblepharismin," Photochemistry and Photobiology 81(6), 1343-1346, (1 November 2005). https://doi.org/10.1562/2005-04-28-RN-504
Received: 28 April 2005; Accepted: 1 May 2005; Published: 1 November 2005
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