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1 March 2006 Time-Resolved Emission Spectra of Green Fluorescent Protein
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Abstract

The time-resolved emission spectra of wild-type green fluorescent protein (wtGFP) and the T203V GFP mutant have been recorded with picosecond time resolution, allowing the separate characterization of the two spectral components associated with the neutral and anionic forms of the GFP chromophore. Significantly, neither component shifts as a function of time. It is suggested that the absence of spectral shift is a result of highly restricted movement of the protein residues in the vicinity of the chromophore. The shapes of the separated spectra are discussed and their relative ratio analyzed in a steady-state analysis.

Andrew A. Jaye, Deborah Stoner-Ma, Pavel Matousek, Michael Towrie, Peter J. Tonge, and Stephen R. Meech "Time-Resolved Emission Spectra of Green Fluorescent Protein," Photochemistry and Photobiology 82(2), 373-379, (1 March 2006). https://doi.org/10.1562/2005-05-07-RA-518
Received: 7 May 2005; Accepted: 1 August 2005; Published: 1 March 2006
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