Translator Disclaimer
1 September 2006 Fluorescence Lifetimes Study of α-Tocopherol and Biological Prenylquinols in Organic Solvents and Model Membranes
Author Affiliations +
Abstract

We have found that for biological prenyllipids, such as plastoquinol-9, α-tocopherol quinol, and α-tocopherol, the shortest fluorescence lifetimes were found in aprotic solvents (hexane, ethyl acetate) whereas the longest lifetimes were those of ubiquinonol-10 in these solvents. For all the investigated prenyllipids, fluorescence lifetime in alcohols increased along with an increase in solvent viscosity. In a concentrated hexane solution, the lifetimes of prenylquinols considerably decreased. This contrasts with methanol solutions, which is probably due to the self-association of these compounds in aprotic solvents. We have also found a correlation of the Stokes shift of prenyllipids fluorescence with the orientation polarizability of the solvents. Based on data obtained in organic solvents, measurements of the fluorescence lifetimes of prenyllipids in liposomes allowed an estimation of the relative distance of their fluorescent rings from the liposome membrane surface, and was found to be the shortest for α-tocopherol quinol in egg yolk phosphatidylcholine liposomes, and increased in the following order: α-tocopherol in dipalmitoyl phosphatidylcholine liposomes < α-tocopherol < plastoquinol-9 < ubiquinol-10 in egg-yolk phosphatidylcholine liposomes.

Jerzy Kruk, Beata Myśliwa-Kurdziel, Małgorzata Jemioła-Rzemińska, and Kazimierz Strzałka "Fluorescence Lifetimes Study of α-Tocopherol and Biological Prenylquinols in Organic Solvents and Model Membranes," Photochemistry and Photobiology 82(5), 1309-1314, (1 September 2006). https://doi.org/10.1562/2006-04-14-RA-872
Received: 14 April 2006; Accepted: 18 June 2006; Published: 1 September 2006
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top