Translator Disclaimer
1 November 2006 Three Putative Photosensory Light, Oxygen or Voltage (LOV) Domains with Distinct Biochemical Properties from the Filamentous Cyanobacterium Anabaena sp. PCC 7120
Author Affiliations +
Abstract

Light, oxygen or voltage (LOV) domains function as blue-light sensors in the phototropin family of photoreceptors found in plants, algae and bacteria. We detected putative LOV domains (Alr3170-LOV, All2875-LOV and Alr1229-LOV) in the genome of a filamentous cyanobacterium, Anabaena sp. PCC 7120. These cyanobacterial LOV domains are closely clustered with the known LOV domains. Alr3170-LOV and All2875-LOV carry the conserved cysteine residue unique to the photoactive LOV, whereas Alr1229-LOV does not. We expressed these three LOV domains in Escherichia coli and purified them. In fact, Alr3170-LOV and All2875-LOV that are conserved in Nostoc punctiforme, a related species, bound flavin mononucleotide and showed spectral changes unique to known LOV domains on illumination with blue light. Alr3170-LOV was completely photoreduced and dark reversion was slow, whereas All2875-LOV was slowly photoreduced and dark reversion was rapid. For comparison, AvAll2875-LOV in a closely related A. variabilis was also studied as a homolog of All2875-LOV. Finally, we observed that Alr1229-LOV that is not conserved in N. punctiforme showed no flavin binding.

Rei Narikawa, Kazunori Zikihara, Koji Okajima, Yuriko Ochiai, Mitsunori Katayama, Yoshinori Shichida, Satoru Tokutomi, and Masahiko Ikeuchi "Three Putative Photosensory Light, Oxygen or Voltage (LOV) Domains with Distinct Biochemical Properties from the Filamentous Cyanobacterium Anabaena sp. PCC 7120," Photochemistry and Photobiology 82(6), 1627-1633, (1 November 2006). https://doi.org/10.1562/2006-05-02-RA-888
Received: 2 May 2006; Accepted: 1 August 2006; Published: 1 November 2006
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top