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1 November 2000 MYO1, a Novel, Unconventional Myosin Gene Affects Endocytosis and Macronuclear Elongation in Tetrahymena thermophila
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Abstract

Targeted gene disruption was used to investigate the function of MYO1, an unconventional myosin gene in Tetrahymena thermophila. Phenotypic analysis of a transformed strain that lacked a functional MYO1 gene was conducted at both 20 °C and 35 °C. At either temperature the Δ MYO1 strain had a smaller cytoplasm/nucleus ratio than wild type. At 20 °C, Δ MYO1 populations had a longer doubling time than wild type, lower saturation density, and a reduced rate of food vacuole formation. However, at 35 °C, these characteristics were comparable to wild type. Although micronuclear division and cytokinesis appeared normal in Δ MYO1 cells, failure of the macronucleus to elongate properly resulted in unequal segregation of macronuclear DNA in cells maintained at either 20 °C or 35 °C.

SELWYN A. WILLIAMS, ROLAND E. HOSEIN, JORGE A. GARCÉS, and R. H. GAVIN "MYO1, a Novel, Unconventional Myosin Gene Affects Endocytosis and Macronuclear Elongation in Tetrahymena thermophila," The Journal of Eukaryotic Microbiology 47(6), 561-568, (1 November 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00090.x
Received: 2 April 2000; Accepted: 31 July 2000; Published: 1 November 2000
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