Negative human–wildlife interactions do not only have adverse effects on rural livelihoods but also lead to negative attitudes toward wildlife conservation. This research uses primary data collected from 221 randomly selected households in the Okavango Delta to analyze their perceptions on poaching and community involvement in anti-poaching activities. The results reveal that the majority of the respondents acknowledge the existence of poaching within their communities. Close to 50% of the respondents noted that they poach for subsistence purposes. There is a generally low participation rate in anti-poaching efforts in the study area. The study concludes that the negative attitudes of communities toward wildlife and wildlife conservation threaten wildlife sustainability in the Okavango Delta. There is a need to strike an intricate balance between wildlife conservation and improving communities’ welfare and tolerance to wildlife through designing effective institutions that are aligned to local realities.