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1 May 2018 Detection of the Trp-2027-Cys Mutation in Fluazifop-P-Butyl–Resistant Itchgrass (Rottboellia cochinchinensis) Using High-Resolution Melting Analysis (HRMA)
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Abstract

Itchgrass [Rottboellia cochinchinensis (Lour.) Clayton] is recognized as one of the most noxious and troublesome annual weeds in tropical and subtropical regions. Acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides have been frequently used for managing R. cochinchinensis POST in a variety of crops, resulting in evolved resistance to these herbicides. Recently, resistance to fluazifop-P-butyl has been demonstrated for this weed, as the result of a G-to-C single-nucleotide polymorphism (SNP) that leads to the Trp-2027-Cys substitution in the ACCase enzyme. This study was conducted to develop a high-resolution melting analysis (HRMA) for the detection of the mutation underlying the Trp-2027-Cys substitution. The HRMA assay allowed differentiating between fluazifop-P-butyl–resistant (C mutant) and susceptible (G wild type) R. cochinchinensis plants. HRMA accuracy was confirmed with DNA sequencing of the target-site mutation, and no false positives or negatives were observed. Our results illustrated how HRMA is effective detecting the Trp-2027-Cys substitution in an R. cochinchinensis resistance, and how this technique can be of great value for developing high-throughput programs for monitoring evolution and dispersion of target site–based herbicide resistance at large scales.

© Weed Science Society of America, 2018. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Walter Barrantes-Santamaría, Rolbin Castillo-Matamoros, Franklin Herrera-Murillo, Arturo Brenes-Angulo, and Luis Gómez-Alpízar "Detection of the Trp-2027-Cys Mutation in Fluazifop-P-Butyl–Resistant Itchgrass (Rottboellia cochinchinensis) Using High-Resolution Melting Analysis (HRMA)," Weed Science 66(3), 286-292, (1 May 2018). https://doi.org/10.1017/wsc.2018.3
Received: 18 August 2017; Accepted: 16 December 2017; Published: 1 May 2018
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