Arrow worms (the phylum Chaetognatha), one of the major marine planktonic animals, exhibit features characteristic to both deuterostomes and protostomes, and their ancestry therefore remains unknown. As the first step to elucidate the molecular bases of arrow worm phytogeny, physiology and embryology, we isolated cDNA clones for three different actin genes (PgAct1, PgAct2 and PgAct3) from the benthic species Paraspadella gotoi, and examined their expression patterns in adults and juveniles. The amino acid sequences of the three actins resembled each other, with identities ranging from 86% to 92%. However, the patterns of the spatial expression of the genes were independent. The PgAct1 gene might encode a cytoplasmic actin and was expressed in oogenic cells, spermatogenic cells, and cells in the ventral ganglion. The PgAct2 and PgAct3 genes encoded actins of divergent types. The former was expressed in well-developed muscle of the head (gnathic) region and trunk muscle cells, whereas the latter was expressed in muscle of the trunk and tail regions and oogenic cells. These results suggest that, similarly to other metazoans, the chaetognath contains multiple forms of actins, which are expressed in various manners in the adult and juvenile arrow worm.
INTRODUCTION
Arrow worms (the phylum Chaetognatha) are about 100 species of marine, largely planktonic animals, with the exception of a few benthic species (cf., Bone et al., 1991). According to Brusca and Brusca (1990), they are bilateral deuterostomes, and are characterized by features including a trimeric body comprised of a head, trunk and postanal tail divided from one another by transverse septa, a body with lateral and caudal fins, a head with a pair of uniquely arranged eyes, and, around the mouth, sets of grasping spines and teeth used for prey capture. Arrow worms possess longitudinal muscles of an unusual type, arranged in quadrants rather than a circular arrangement, a complete gut anus at the ventral surface of the trunk-tail junction, and a central nervous system including a cerebral ganglion in the dorsal side of the head and a large ventral ganglion in the trunk. These animals are hermaphroditic and direct developers.
The phylogenetic status of chaetognaths is mysterious. They share some common characteristics with deuterostomes during their ontogeny; radial cleavage, a blastopore at the rear end of the body, and a postanal tail (Hyman, 1959; Brusca and Brusca, 1990; Willmer, 1990). However, the morphology of adults - namely, a coelom without a peritoneum and the apparent lack of circular muscle in the body wail - suggests their similarity to pseudocoelomate groups (Willmer, 1990). although their coelomic condition is still debated (Shinn and Roberts, 1994). Their nervous system is more like that of protostomes (Rehkämper and Welsch, 1985; Goto anc Yoshida, 1987). Moreover, even during ontogeny, they do not pass through the dipleurula stage that is seen in a few deuterostome phyla. Recent molecular phylogenetic studies using 18S rDNA (rRNA), sequences did not support the affinity of chaetognaths with deuterostomes (Telford and Holland, 1993; Wada and Satoh, 1994).
Despite such characteristics and their phylogenic position, arrow worms have been a subject of very few molecular biological investigations. Our laboratory at the Mie University has recently succeeded in the maintenance of the benthic species Paraspadella gotoi by extending several generations P. gotoi may provide an appropriate experimental system to investigate the molecular bases of the physiology, behavioral biology and developmental biology of the arrow worm. As the first step to elucidate the molecule basis, the present study was performed to isolate cDNA clones for actin genes from P gotoi. Actin is a ubiquitous protein that is encoded by a multigene family in a variety of animals (Pollard and Cooper 1986; Rubenstein, 1990). There are two subtypes of actin muscle type and cytoplasmic type (Vandekerckhove and Weber, 1984). Several kinds of muscle-type actin mRNA have also been shown in certain higher eukaryotes. The coding sequences of different members of the actin gene family are conserved, whereas the 5′ and 3′ non-coding sequences usually diverge and can be used as gene-specific probes (Shani et al., 1981). Each actin isoform shows a distinct expression pattern specific to tissues or developmental stages (Sanchez et al., 1983; Schwartz and Rothblum, 1981; Kusakabe et al., 1995). The present study revealed that the arrow worm also contains multiple forms of actin, which show different patterns of spatial expression.
MATERIALS AND METHODS
Animals
Paraspadella gotoi Casanova (Casanova, 1990) was collected in the vicinity of Amakusa Marine Biological Station, Kyushu University, Kumamoto, Japan. P. gotoi belongs to the order Pharagmorpha with ventral transverse muscle bands (pharagma) and is a benthic species. Adults specimens have been maintained in our laboratory at Mie University in a constant-temperature room at 17°C and fed with Tigriopus japonicus. They are cross-fertile and fertilization occurs internally. Juveniles were obtained by collecting laid eggs and were kept in a constant-temperature room at 23°C. The young hatched 2 days after egg-laying. Details of the culture method have been described elsewhere (Goto and Yoshida, 1997).
Isolation of RNA and construction of a cDNA library of P. gotoi adult
Total RNA was isolated from whole adult specimens (about 300 individuals) by the acid guanidinium thiocyanate-phenol-chloroform (AGPC) method (Chomczynski and Sacchi, 1987). Poly(A)+ RNA was purified by use of Oligotex-dT30 Latex beads (Roche Japan, Tokyo) according to the manufacturers protocol. Complementary DNA was synthesized from the poly(A)+ RNA with a Zap cDNA Synthesis kit (Stratagene, La Jolla, CA, USA). Double-stranded cDNA was size-fractionated on a column of Sephacryl S-500 (Pharmacia Biotech, Uppsala, Sweden), and fractions that contained fragments more than 300 bp in length were collected. The double-stranded cDNA was cloned directly into the EcoRl-Xhol site of a Uni-ZAPXR vector (Stratagene). The titer of the amplified cDNA library was estimated to be2.4×107pfu/μl.
Isolation and sequencing of cDNA clones for P. gotoi actin genes
The amino acid sequences of actins are highly conserved among eukaryotes (e.g., Kusakabe et al., 1997). The primers were designed as follows: muscle forward, 5′-TG(C/T)GA(C/T)AA(C/T)GG(A/C/G/T)(A/T)(C/G)(A/C/G/T)GG(A/C/G/T)(C/T)T-3′; cytoplasmic forward, 5′-GT(A/C/G/T)GA(C/T)AA(C/T)GG(A/C/G/T)(A/T)(C/G)(A/C/G/T)GG(A/C/G/T)ATG-3′; and actin reverse, 5′-AA(A/G)CA(C/T)TT(A/C/G/T)C(G/T)(A/G)TG(A/C/G/T)AC(A/G/T)AT-3′. Using these oligonucleotide primers, we amplified target fragments from the first-stranded cDNAs which were obtained from RNAs of P. gotoi by means of reverse transcription-polymerase chain reaction (RT-PCR). Annealing was carried out at 37°C or at 42°C. Sequencing revealed that the amplified fragments were of actin genes.
Probing with candidate fragments random-labeled with [32P]-dCTP (Amersham, Buckinghamshire, UK), we screened the cDNA library at moderate stringency conditions (hybridization; 5× SSPE, 0.5% SDS, 5× Denhardt's solution, 35% formamide at 42°C: washing; 2× SSC, 0.1% SDS at 37°C for 30 min twice and at 42°C for 30 min once: Sambrook et al., 1989) and obtained many positive clones.
The nucleotide sequences were determined for both strands with a dye primer cycle sequencing FS ready kit and ABI PRISM 377 DNA sequencer (Perkin Elmer, Norwalk, CT, USA).
In situ hybridization
In situ hybridization was carried out with both whole-mount specimens of newly-hatched juveniles and sectioned specimens of adults. All specimens were fixed in 4% paraformaldehyde in MOPS buffer (pH 7.5), 0.5 M NaCI. Fixed specimens were immersed in 80% ethanol and kept at -20°C until use. The probes were synthesized from the 3′ untranslated region of the gene by following the instructions from the supplier of the kit (DIG RNA Labeling kit; Boehringer Mannheim, Mannheim, Germany).
Whole-mount specimens: After a thorough washing with PBT [phosphate-buffered saline (PBS) containing 0.1% Tween 20], the fixed specimens were treated with 2 μg/ml proteinase K (Merck, Darmstadt, Germany) in PBS for 20 min at 37°C, and then they were post-fixed with 4% paraformaldehyde in PBS for 1 hr at room temperature. After a 1-hr period of prehybridization at 42°C, the specimens were allowed to hybridize with the digoxigenin-labeled antisense or sense probe for at least 16 hr at 42°C. After hybridization, the specimens were washed and treated with RNase A and then washed again extensively with PBT. The samples were then incubated for 1 hr with 1:2000 Boehringer Mannheim alkaline-phosphatase-conjugated anti-DIG and treated for the development of color as indicated in the protocol from Boehringer. After dehydration, some of the specimens were cleared by placing them in a 1:2 mixture (v/v) of benzyl alcohol and benzyl benzoate.
Sectioned specimens: These specimens were embedded in polyester wax and sectioned at 10 μm. The In situ hybridization of sectioned specimens was carried out as in the case of whole-mount specimens.
RESULTS AND DISCUSSION
Isolation and characterization of a cDNA clone for Pamspadella gotoi actin genes
We first made first-stranded cDNAs against P. gotoi adult RNAs. Then, using oligonucleotide primers corresponding to the shared amino-acid sequences of actins, we amplified target fragments from them by the RT-PCR reaction. We obtained 12 amplified fragments which were about 1.1-kb long and corresponded to the region from the amino acid position 10 to 375 of actins. Sequencing the fragments after subcloning them into pBluescript II SK(+) revealed that these 12 clones were subdivided into three types (Types-1, -2, and -3); 6 clones were of Type-1, 3 were of Type-2, and 3 were of Type-3.
With labeled clones, we screened 2.4 × 105 pfu of the P. gotoi adult cDNA library and obtained 42 positive clones. The partial sequencing of these clones revealed that 10 contained sequences identical to Type-1, 5 to Type-2, and 6 to Type-3. In addition to these three types, there were seven different types of 12 clones, which were not analyzed further. Of the clones belonging to each of the three types, the longest ones were entirely sequenced. Each of the longest clones contained a single open reading frame (ORF) that predicted actins. We therefore named the corresponding genes PgAct1 (Paraspadella gotoi actin gene 1) to Type-1, PgAct2 to Type-2, and PgAct3 to Type-3.
Figure 1 shows the nucleotide and deduced amino acid sequences of the cDNA clone for the PgAct1 gene, which consisted of 1,677 nucleotides. The PgAct1 cDNA contained a single ORF of 1,128 bp, which predicted a polypeptide of 376 amino acids. The calculated molecular mass of the predicted protein was 41.8 kDa.
The nucleotide and deduced amino acid sequences of the cDNA clone for the PgAct2 and PgAct3 genes are shown in Figs. 2 and 3. The PgAct2 cDNA was 1,534 bp long and contained a single ORF of 1,128 bp, which predicted a polypeptide of 377 amino acids and had a calculated molecular mass of 42.3 kDa, while the PgAct3 cDNA was 1,510 bp long and contained a single ORF of 1,128 bp, which predicted a polypeptide of 377 amino acids and 42.3 kDa.
Among these three actins, the amino acid identities were 91.5% between PgAct1 and PgAct2, 86.2% between PgAct1 and PgAct3, and 88.9% between PgAct2 and PgAct3, although the identity of the nucleotide sequences of 3′ untranslated region (UTR) was less than 50%.
Figure 4 shows a comparison of the amino acid sequences of PgAct1, PgAct2 and PgAct3 with actins of various metazoans. The comparison clearly indicated that the PgAct1, PgAct2, and PgAct3 genes encode different types of actin. As shown in Fig. 4, the mammalian α-striated muscle actin is distinguishable from the β-cytoplasmic actin by a comparison of amino acid residues at the diagnostic positions (Vandekerckhove and Weber, 1978, 1984). Figure 4 suggests that PgAct1 is of a type of cytoplasmic actin and that PgAct2 and PgAct3 are considerably divergent types of actin. Our molecular phylogenetic analysis comparing the amino acid sequences also suggested that PgAct2 and PgAct3 are closely related, while PgAct1 is distinct from them, that PgAct1 is a type of cytoplasmic actin, and that PgAct2 and PgAct3 are divergent types of actin (Yasuda et al., unpublished data).
These results suggest that the genome of P. gotoi contains a family of multiple actin genes and that among them, PgAct1, PgAct2, and PgAct3 are major actin genes judging from the numbers of cDNA clones isolated. Because 6 of the 12 amplified fragments and 10 of the 42 positive cDNA clones corresponded to PgAct1, this gene may represent the most dominant actin gene of P. gotoi. PgAct2 and PgAct3 genes are also actively expressed in this animal, judging from the numbers of cDNA clones obtained.
Expression of Paraspadella gotoi actin genes
The expression of the arrow worm actin genes was examined by In situ hybridization with sectioned specimens of adults and whole-mount specimens of juveniles. To help clarify the gene expression, the anatomy of P. gotoi is diagrammatically shown in Fig. 5a. Their trimeric body is comprised of a head, trunk and postanal tail divided from one another by transverse septa. The head cavity is reduced by the complex cephalic musculature. The body musculature consists of four quadrants of well-developed dorsolateral and ventrolateral longitudinal bands. The digestive tract runs through the central part of the trunk region and is completed by the anus at the ventral surface of the trunk-tail junction. A major component of the central nervous system is a large ventral ganglion located in the trunk epidermis. Arrow worms are hermaphroditic; the wide coelom of the trunk region is occupied with oogenic cells, while that of the tail region occupied by spermatogenic cells. Adults were examined in cross-sections of the head, trunk, and tail regions.
The PgAct1 gene.
The In situ hybridization of adult specimens demonstrated intense signals in oogenic cells of the trunk region (Fig. 5c). Small oocytes showed stronger signals, whereas large mature oocytes showed weak signals (Fig. 5c). In addition, sections of the tail region revealed signals in the spermatogenic cells or spermatocytes (Fig. 5d). Therefore, the PgAct1 gene is expressed in germ cells during the early phase of their formation.
Hybridization signals were also evident in the cytoplasm of the neuronal cells of the ventral ganglion (Fig. 5c) as well as in cells of the digestive tract including the pharynx in the head region (Fig. 5b) and intestine in the trunk region (Fig. 5c). However, signals were not so evident in longitudinal muscle cells (Fig. 5c, d).
The In situ hybridization of the whole-mount specimens of newly-hatched juveniles showed intense signals on the whole body except for the tail region (Fig. 5e). Strong signals were seen in the head region (Fig. 5e).
These results suggest that the PgAct1 gene is expressed both maternally and zygotically. The gene is expressed in a variety of tissues, supporting the notion that PgAct1 is a type of cytoplasmic actin.
The PgAct2 gene.
Cross-sections of the head region of the adults demonstrated that the PgAct2 gene was actively expressed in well-developed muscle of the gnatha region (Fig. 6a). Hybridization signals were also evident on the epithelium lining the coelom near the ventral ganglion (Fig. 6b) and in cells of the sperm duct of the trunk region (Fig. 6b). However, signals were not detected in the pharynx (Fig. 6a, b) or germ cells (Fig. 6b, c).
The In situ hybridization of whole-mount specimens of newly-hatched juveniles showed intense signals in the head region, in particular in the gnathic region (Fig. 6d). Lateral cells in the trunk region also showed signals (Fig. 6d).
These results suggest that the PgAct2 gene expression is zygotic, and primarily found in muscle cells.
The PgAct3 gene.
In contrast to the PgAct2 gene, the In situ hybridization of sectioned specimens of adult tissues demonstrated intense signals in oogenic cells of the trunk region (Fig. 6f). The small oocytes showed very strong signals (Fig. 6f). Signals were also evident in longitudinal muscle cells of the trunk and tail regions (Fig. 6f, g). In the tail region, signals were evident in the mesentery (Fig. 6g). The In situ hybridization of whole-mount specimens of newly-hatched juveniles showed intense signals on the whole body (Fig. 6h).
These results suggest that the PgAct2 gene is primarily expressed in the longitudinal muscle as well as oocytes.
The regions with expressions of PgAct1, PgAct2 or PgAct3 are summarized and compared in Table 1. As is evident in the table, the patterns of spatial expression of the three actin genes are not identical but rather are specific for each gene. It is highly likely that each of these genes has its own function.
Table 1
Spatial expression of the three actin genes PgAct1, PgAct2, and PgAct3 of the arrow worm Paraspadella gotoi
Acknowledgments
We thank Dr. T. Kusakabe for his help with molecular phylogenetic analysis. This research was supported in part by a Grant-in-Aid for Specially Promoted Research (No. 07102012) from the Ministry of Education, Science, Sports and Culture of Japan to N.S.