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1 September 2003 Endocrine Control of the Reproductive Activity in Hibernating Bats
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Abstract

Bats, Chiroptera, constitute the second largest order of the class Mammalia and vary greatly in habitats, available foods and mating systems. The timing, duration and patterns of reproduction in bats vary considerably among species and different localities. Though much is known about the reproductive phenomena and associated endocrine characteristics of various species, the central mechanism regulating the peculiar delay and asynchrony in reproductive activity remains to be elucidated. The current understanding on the endocrine characteristics and possible mechanism of regulation of the hypothalamo-adenohypophysial-gonadal axis of bats will be reviewed, based mainly on our own studies in hibernating rhinolophid bats.

INTRODUCTION

Bats, Chiroptera, constitute the second largest order of mammals next to rodents (about 960 species in the world). In the tropical region, they are extremely abundant in the number of species and their population probably exceed that of rodents. Bats also vary greatly in terms of habitat, available foods (nectar to blood) and mating systems (Altringham, 1996; McCracken and Wilkinson, 2000). In general, although echo location and hibernation are well known in bats as well as some species of mammals, unique reproductive patterns are also documented in bats (Gustafson, 1979; Oxberry, 1979; Racey and Entwistle, 2000). One unusual reproductive feature is the delay of ovulation and fertilization. This phenomenon is not confined to bats but it has been found in several species of rodents. A second feature is the delayed implantation of the embryo. This is popular among numerous mammalian species, particularly in carnivores (Clarke, 1981). The retention time of viable spermatozoa within the female reproductive tract is very limited. The mature follicle is not maintained for long period in the ovary, and after the maturation the follicle is ovulated naturally or through the stimulation of copulation. These reproductive patterns with interruption for a long period by hibernation are characteristic to bats inhabiting the temperate zones, the pattern not occurring in other mammalian hibernators. A third unique feature is the delayed development of embryo. This phenomenon is observed in bats living in the tropical and subtropical zones (Fleming, 1971; Heideman, 1989).

In the temperate zone, most species of bats are insectivorous and display a restricted seasonal monoestry with copulation occurring in autumn. In males of most species, spermatogenesis peaks in summer, but mating behavior and the maximal development of accessory organs are delayed until autumn (Gustafson, 1979; Racey and Entwistle, 2000). Female bats hibernate with spermatozoa stored in their reproductive tracts; oviducts in most species (delayed ovulation/fertilization type) or at the early pregnant stage with unimplanted blastocysts in their uteri (delayed implantation type) for about five months until the next spring. After arousal in spring, ovulation/fertilization and implantation occur in bats exhibiting the delayed ovulation and implantation patterns, respectively (Oxberry, 1979). Female bats form a maternity colony and begin parturition (monotocy in most species) and lactation during summer. This timing coincides with peak food availability, which is likely to maximize reproductive success. After weaning, mother bats take food to reserve energy in the body, mostly in the form of fat, for the preparation for the next hibernation. Before hibernation, mature female bats enter estrus and copulate with males. Thus, bats inhabiting the temperate zone show a unique reproductive activity prior to hibernation (Racey, 1982), which attracted much attention of the investigators. However, the reproductive phenomena and associated endocrine function have been documented in a few species, and the majority of such studies are, if any, fragmentary. The aim of this article is to review the present status of research on reproductive function including our own findings in rhinolophid bats.

Annual reproductive cycle

Annual reproductive cycle of bats inhabiting the temperate zones fundamentally follows the two patterns, delayed ovulation/fertilization and delayed implantation (Fig. 1). In the islands of Japan, Japanese house bats, Pipistrellus abramus (Vespertilionidae), horseshoe bats, Rhinolophus ferrumequinum (Rhinolophidae) and Myotis macrodactylus (Vespertilionidae) exhibit the former type (Uchida and Mōri, 1987) (Fig. 1A). In male bats corresponding to this pattern of females, a seasonally separated asynchrony between testicular function and mating behavior is noted. The latter pattern is found in certain species of the vespertilionid genus Miniopterus and rhinolophid genus Rhinolophus (Oxberry, 1979). A long-fingered bat, Miniopterus fuliginosus (Vespertilionidae) exhibits the latter pattern (Fig. 1B). Reproductive cycles of other species living in Japan remain unknown.

Fig. 1

Annual reproductive cycle of bats showing delayed ovulation/fertilization in females and the corresponding pattern in males (A), and delayed implantation in females and the corresponding pattern in males (B). TR: testicular regression. Modified from Kawamoto et al., 1998 and Oxberry,1979.

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Hypothalamus

Seasonal changes of the hypothalamic neuropeptides in mammalian hibernators, especially the changes of neuropeptide-producing cells associated with hibernation, have been reported in hedgehogs, hamsters, dormice and ground squirrels (Nürnberger, 1995). Though there are several reports on the hypothalamic neuropeptides in bats (Mikami et al., 1988b; Anthony et al., 1991), detailed analyses have only been performed on gonadotropin-releasing hormone (GnRH). GnRH is the key hypothalamic hormone responsible for the regulation of reproductive function. GnRH stimulates the pulsatile release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from gonadotrophs in the pars distalis. Although the amino acid sequence of this decapeptide is basically conserved among mammalian species, the presence of a variant with three amino acid substitutions (chicken GnRH II) is documented in several metatherian and early-evolved eutherian species. However, only the mammalian GnRH is identified in the bat (Myotis lucifugus) brain (King et al., 1994).

In seasonal breeding mammals, gonadal activity depends on environmental factors, especially photoperiod. In long-day breeding rodents, such as hamsters, animals exposed to short-day photoperiods in captivity or to naturally declining day lengths show a decline in gonadal function concomitant with a decrease in the release of hypothalamic GnRH (Pickard and Silverman, 1979; Pieper, 1984; Glass, 1986; Kriegsfeld and Nelson, 1999; Kawamoto et al., 2000b) and gonadotropins (Turek et al., 1975; Tamarkin et al., 1976; Pickard and Silverman, 1979; Simpson et al., 1982; Yellon and Goldman, 1987; Niklowitz et al., 1989). In mammals, the effect of photoperiod is mediated by the nocturnal secretion of melatonin from the pineal gland, but the exact mechanism of neuroendocrine regulation by which photoperiod causes physiological adjustments remains unknown (Malpaux et al., 2001).

In rodent species, hypothalamic GnRH neuronal cell bodies are preferentially distributed rostrally, within the diagonal band of the Broca, the preoptic area and the anterior hypothalamic area, particularly in the medial preoptic area which is the major source of GnRH neuronal terminals in the median eminence, the release site of GnRH to the adenohypophysis (Silverman et al., 1979; Jennes and Stumpf, 1980; Witkin et al., 1982). In contrast, the majority of GnRH neuronal perikarya in vespertilionid bats, Myotis lucifugus (King et al., 1984), M. macrodactylus (Kawamoto, unpublished data), Miniopterus shreibersii (Mikami et al., 1988b; Fernández et al., 1992) and Eptesicus fuscus (Oelschläger and Northcutt, 1992) are located in the medial basal hypothalamus, particularly in the region of the arcuate nucleus, and their distribution well resembles that in higher primates (Parker et al., 1980; Silverman et al., 1982). These GnRH neurons are fusiform-shaped or bipolar with a smooth outline in bats. Further analyses of the GnRH neuronal system in relation to the season and reproductive function have only been documented in a few species of bats (Anthony et al., 1989; Fernández et al., 1992). We have studied seasonal changes and sex difference in the hypothalamic GnRH neurons of rhinolophid bats, R. ferrumequinum (Kawamoto et al., 1998). Although GnRH-immunoreactive neurons in this species were encountered in the area from the diagonal band of the Broca to the mammillary body, they were concentrated in the medial preoptic area and medial basal hypothalamus. This distribution seems to be a characteristic feature of rhinolophid bats, which is not found in vespertilionid bats. In rhinolophid bats, the seasonal changes in the number and immunoreactivity of GnRH neurons were greater in the medial basal hypothalamus than in the medial preoptic area. These results suggest that the GnRH neuronal activity in the medial basal hypothalamus is more closely associated with the reproductive function of this bat than that in the medial preoptic area. Furthermore, alterations of GnRH-immunoreactive neurons in the medial basal hypothalamus are closely related with the density of GnRHimmunoreactive fibers in the median eminence. In vespertilionid bats, numbers of GnRH-immunoreactive neurons in the medial basal hypothalamus also vary depending on the reproductive state (Anthony et al., 1989; Fernández et al., 1992; Anthony, 2000). These findings, therefore, suggest that GnRH neurons located in the medial basal hypothalamus are the main perikarya of GnRH terminals in the median eminence and play a central role in the seasonal changes of gonadotropin secretion in both rhinolophid and vespertilionid bats. Our study in rhinolophid bats revealed a sex difference in the number of GnRH neurons in the medial preoptic nucleus in early June to late July. The number of immunoreactive neurons located in the medial preoptic nucleus was significantly less in females than males. From May to August, pregnancy, parturition and lactation occur in mature female bats, and therefore enhanced GnRH neuronal activity in the medial preoptic area may be related to female-specific reproductive events.

King et al. (1984) reported in female vespertilionid bats (M. lucifugus) that GnRH neurons innervate not only to the median eminence but also the pars nervosa. They pointed out that immunoreactive GnRHs in the pars nervosa were chemically identical with those in the hypothalamus (Anthony et al., 1987) and immunoreactivity was significantly reduced in the pars nervosa as well as in the hypothalamus and median eminence during periovulatory period (Anthony et al., 1989). These parallel reduction of GnRH associated with ovulation suggests that the neural lobe component of the system contributes to the control of gonadotropin secretion in this bat. These findings indicate that multiple release sites may be present in the median eminence, infundibular stalk and neural lobe for the delivery of GnRH to the bat anterior pituitary (Anthony et al., 1984, 1989). However, the innervation of GnRH terminals into the pars nervosa has not been reported in other species of bats.

Adenohypophysis

The adenohypophysis is divided into the pars distalis, pars intermedia and pars tuberalis. However, to our knowledge there are no reports on the pars intermedia in bats.

(1) Pars Distalis

As in other mammalian species, six kinds of hormones and five kinds of hormone-secreting cells exist in the pars distalis of bats. Most findings on the function of hormone-producing cells have been made using immunohistochemistry with heterologous antisera as follows. During the arousal period, thyrotrophs show the increased activity (Anthony and Gustafson, 1984b). Annual changes in corticotrophs are related to the so-called fat cycle in tropical vespertilionid bats (Singh and Krishna, 1996b). In vespertilionid bats inhabiting the temperate zone, there were no seasonal variations in somatotrophs (Anthony et al., 1991). In several species of bats inhabiting the temperate zone or tropical zone, immunohistochemical studies have also verified changes in mammotrophs (lactotrophs) relating to reproductive cycle and seasonality (Richardson, 1981b; Jemenez et al., 1987; Mikami et al., 1988a; Ishibashi and Shiino, 1989; Muñiz et al., 1991; Singh and Krishna, 1996a). In rhinolophid bats, grandular cells showing proliferative activity in the pars distalis were identified as mammotrophs in both sexes (Fig. 2). Mammotrophs occupy more hypophysial area in early summer (pregnant period) and mid-summer (lactation period) than in other seasons, suggesting that hypophysial prolactin (PRL) contents in female bats were elevated during these periods (Fig. 2A). In the male the seasonal variation is not distinct. Similar changes have been reported in several species of vespertilionid bats (Jemenez et al., 1987; Mikami et al., 1988a; Singh and Krishna, 1996a). Proliferative activity of mammotrophs in females was detected in early summer (pregnant period) and midsummer (lactating period), but not in other seasons (Fig. 2B). In male bats, this activity was low and continued throughout the year except in winter (hibernation period). It has been well established that a direct estradiol action on mammotrophs is required for the hypertrophy and proliferative activity of these cells (e.g. Lloyd et al., 1975), indicating increased steroidogenesis in female bats during summer.

Fig. 2

Seasonal changes of PRL-immunoreactive (ir) cells (mammotrophs) in the pars distalis of adult bats, R. ferrumequinum. (A) Percentage of area occupied by PRL cells. (B) Number of PRL-ir cells showing proliferative activity per unit area. The section was doubly immunostained with anti-ovine PRL and anti-proliferating cell nuclear antigen (PCNA) sera. P: pregnant period (mid-June), R: recrudescent period (mid-June), L: lactating period (late July), S: spermatogenic period (early August), M: mating period (mid-October), H: hibernating period (January). Each column and vertical line represent the mean ± SEM. Data with different letters above the columns are significantly different from one another, p<0.05. Asterisks represent significant differences between males and females, p<0.05.

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The presence of mammosomatotrophs, bihormonal cells that secrete both PRL and growth hormone (GH), has been well documented in the pars distalis of various mammals during fetal, pregnant and lactating periods, and in human pituitary adenomas (Mulchahey and Jaffe, 1988; Kineman et al., 1991; Nikitovitch-Winer et al., 1987; Li et al., 1993). Ishibashi and Shiino (1989) have demonstrated the presence of mammosomatotrophs in the pars distalis of Japanese house bats (P. abramus, Vespertilionidae) by immunoelectron microscopy. Based on their findings, mammosomatotrophs were classified into the following four cell types: 1) only mixed granules containing both PRL and GH, 2) mixed and PRL granules, 3) mixed, PRL and GH granules, and 4) mixed and GH granules. These cells exist throughout the annual cycle, while mammotrophs containing pure PRL granules appear only during pregnancy and lactation. From these findings, it was concluded that mammosomatotrophs, which constantly exist throughout the annual cycle, probably function as stem cells which have the potential to differentiate into mammotrophs. In rhinolophid bats, though mammosomatotrophs in female hypophysis were detected during lactation and pregnancy, mammotrophs containing only PRL were also observed in all seasons (Kawamoto, unpublished data). Thus, the appearance of pure PRL cells relating to reproductive function in female bats shows species variation. Though the role of mammosomatotrophs is not completely understood, several attempts have been made to explain their physiological significance (Takahashi, 1995).

Owing to a lack of purified bat adenohypophysial hormones and specific antisera against these hormones, there have been only a few reports on the blood level of hypophysial hormones in the bat. O'Brien et al. (1996) measured plasma PRL concentrations in male and female megachiropteran bats using a heterologous assay. They reported that circulating PRL levels and hypophysial PRL contents were higher in females than males, and that these levels were high during pregnant and lactating periods, declining as breeding began in autumn. These findings are consistent with the results of previous immunohistochemical studies (Jemenez et al., 1987; Mikami et al., 1988a). From the fact that circulating PRL levels are influenced by dopaminergic agents, O'Brien et al. (1996) further concluded that PRL levels play roles in the reproductive physiology of bats, as in other mammalian species, under dopaminergic regulation.

On the other hand, close relationship between circulating PRL levels and delayed implantation in vespertilionid bats is discussed by Anthony (2000). Mammmotrophic activities and PRL levels were constantly low during follicular development and the early period of delayed implantaion, and these functions and levels increased when the delay came to an end and implantation occurred (Anthony, 2000). According to Bernard and Bojarski (1994), the exogenous treatment of PRL induces the onset of implantation in pregnant female bats, M. schreibersii showing delayed implantation pattern, which results in the elevated progesterone (P) levels. While, human chorionic gonadotropin stimulates luteal steroidgenesis, but did not induce implantaion. These results may show that elevated synthesis and secretion of PRL activate the corpus luteum and induce the initiation of implantation (Bernard and Bojarski, 1994).

Seasonal changes of gonadotrophs associated with the annual reproductive cycle have been studied by immunocytochemistry in many species of bats (Richardson, 1979, 1981a; Anthony and Gustafson, 1984a; Anthony, 1987; Mikami et al., 1988a; Bernard et al., 1991ab; Singh and Krishna, 1996a; Kawamoto et al., 2000a). Our studies of rhinolophid bats of both sexes verified that the immunoreactivity and size of LHβ- and FSHβ-immunoreactive cells varied significantly throughout the seasons (Fig. 3A–D). These seasonal variation is considerably greater in females than in males (Fig. 4) as pointed out previously (Anthony, 1987). In reproductively active periods (August), both LHβ- and FSHβ-immunoreactive cells became hypertrophied and showed weaker immunoreactivity. In female bats, FSHβ-immunoreactivity became undetectable during the pregnant period (mid-June), suggesting the need of FSH (Fig. 3A). In contrast to pregnant and lactating periods, in the mating season (mid-October) FSHβ-immunoreactivity and cell size returned to near winter values (hibernation period, January) (Figs. 3C, D and 4). Such a reciprocal relation between the immunoreactivity and cell size of gonadotrophs in both sexes may reflect seasonal differences in the dynamics of secretion, storage and synthesis of these hormones during the annual reproductive cycle. These findings are consistent with previous results in other species of bats (Anthony and Gustafson 1984a; Bernard et al., 1991a; Mikami et al., 1988a; Singh and Krishna, 1996a). Such analyses are useful for evaluating the functional status of gonadotrophs in the pars distalis, but do not yield information on the variation in the rate of hormonal secretion and in the responsiveness to secretagogues.

Fig. 3

Photomicrographs of FSHβ-ir cells in the pars distalis (horizontal plane) of female adult bats (A–D) and LHβ-ir cells in the pars tuberalis (frontal plane) of male adult bats (E-H). The section was immunostained with anti-rat FSHβ serum or anti-rat LHβ serum. A : pregnant period (mid-June), B: lactating period (late July), C and G: mating period (mid-October), D and H: hibernating period (January), E: recrudescent period (mid-June), and F: spermatogenic period (early August). ME: median eminence. Each bar represents 20 μm. Arrows in B indicate weakly stained FSHβ-ir cells. Note the absence of cells immunostained with antisera in A and G.

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Fig. 4

Seasonal changes in cell size and immunoreactivity in the pars distalis of female and male adult bats. ND: no detection. Other explanations are the same as for Fig. 2.

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Substances secreted from endocrine cells can be detected at the single cell level using a reverse hemolytic plaque assay (Smith et al., 1986) or cell immunoblot assay (Arita, 1993). The cell immunoblot assay can sensitively examine not only the incidence of secreting cells in the total cell population, but the amount of substances secreted from a single cell or from a cell population. The function of gonadotrophs in the pars distalis is controlled by the action of hypothalamic GnRH, sex steroids and glycoprotein hormones of gonadal origin. Then, we examined seasonal changes in the gonadotropin secretion and the responsiveness of gonadotrophs to GnRH in male rhinolophid bats by employing the method described above (Fig. 5). We found that the seasonal variations in the total amount of gonadotropins secreted from gonadotrophs per unit volume of chamber were considered due to the changes in gonadotropin secretion per cell and in the number of gonadotropin-secreting cells (Kawamoto et al., 2000a). The total amount of LH secreted from gonadotrophs was marked in spermatogenic and mating periods but extremely small in other phases (post-arousal period, mid-May, and hibernation period, late January). Changes in the total amount of FSH secreted were similar to those in LH secretion. GnRH significantly increased the total amount of gonadotropin secreted from gonadotrophs throughout the annual reproductive cycle except for LH in the post-arousal period and FSH in the hibernation period. In particular, the effect of GnRH on the secretion of both hormones was markedly enhanced during the spermatogenic period. Thus, the responsiveness of gonadotrophs to GnRH also shows seasonal variations, which may be closely associated with the number of GnRH receptors on gonadotrophs and/or with the increased response of gonadotrophs to GnRH caused by the action of gonadal steroids (Conn, 1994; Turzillo et al., 1998; Kawakami and Winters, 1999). In the hibernation period, the majority of gonadotrophs were composed of non-releasable cells but a slight secretory response of gonadotrophs to GnRH was detected at low incubation temperature (10°C, Kawamoto et al., 2000a). These results suggest that the GnRH receptor and subsequent post-receptor events that regulate the action of GnRH on gonadotropin secretion do not completely disappear under heterothermic conditions during hibernation. The secretion of FSH is controlled by glycoproteins (inhibin, activin and follistatin) of gonadal origin in addition to hypothalamic GnRH. However, there are no data available on the effects of these glycoproteins for FSH secretion in bats.

Fig. 5

Seasonal changes in the total amount of gonadotropin secreted from gonadotrophs per unit area of the transfer membrane. A enzymatically dissociated cell suspension was collected from the pars distalis of male adult bats and incubated for 3 hr at 37°C in an atmosphere of 95% air and 5% CO2. PA: post-arousal period (midMay). Asterisks represent significant differences between control and stimulated groups, p<0.05. Other explanations are the same as for Fig. 2. Reprinted from Kawamoto et al. (2000a) with permission from Wiley-Liss, Inc.

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By radioimmunoassay using antiserum to ovine LH, Bernard et al. (1991b) have reported the changes in circulating LH levels during delayed implantation in long-fingered female bats (M. schreibersii). Although this cosmopolitan species usually hibernates during winter in temperate zones (Mikami et al., 1988a; Fernández et al., 1992), the species in South Africa studied by Bernard et al. (1991b) remains active and enters daily torpor only during very cold periods. They found that plasma LH levels were the highest during follicular development and peaked just before ovulation (April), suggesting that the LH surge is required for the initiation of ovulation as in other mammalian species. Plasma levels then dropped rapidly during early delayed implantation (May), beginning to rise again prior to the end of the delay period (August). After implantation, LH levels continued to increase, again reaching a peak in late pregnancy (November). This peak coincides with the maximal level of circulating plasma P throughout the year. Thus, these findings clearly demonstrate that there are close relationships between low LH levels and delayed implantation, resulting in the inhibition of luteal activity during delayed implantation.

(2) Pars Tuberalis

The pars tuberalis of the adenohypophysis covers both the median eminence and the infundibular stalk as thin layers of cells, which is a main target site for melatonin secreted by the pineal gland (Vanecek, 1988; Wittkowski et al., 1999) and may be involved in photoperiodic regulation of endocrine systems in seasonally breeding mammals (Goldman and Darrow, 1983; Hazlerigg et al., 2001; Malpaux et al., 2001). The cells in the pars tuberalis are usually classified into three types, that is, pars tuberalis-specific cells, folliculo-stellate cells and granular endocrine cells (Wittkowski et al., 1999; Hazlerigg, 2001). In several mammals, most of the granular endocrine cells in the pars tuber-alis can be identified as gonadotrophs and thyrotrophs (Hazlerigg, 2001). However, there is little information available about the functional roles of these cells and the regulatory mechanism of their hormonal secretion.

We found that gonadotrophs (FSHβ- or LHβ-immnuno-reactive cells) were distributed throughout the pars tuberalis in hamsters. However, gonadectomy in hamsters induced the hypertrophy and decreased the immunoreactivity of gonadotrophs in the pars distalis, whereas no alterations were detected in gonadotrophs of the pars tuberalis. By employing Western blotting and radioligand binding assay, we furthermore clarified that the concentrations of estrogen receptors and GnRH receptors were far lower in the pars tuberalis than in the pars distalis. Removal of the negative feedback action of estradiol produces a well-known increase in gonadotropin secretion and gonadotropin β-subunit mRNA levels, and resulted in considerable hypertrophy of gonadotrophs in the pars distalis. Such a negative feedback action of estradiol in the regulation of gonadotrophs is generally thought to be an indirect effect through the hypothalamus, by modifying GnRH pulse generation, because estradiol has no inhibitory effects on FSH and LH release from gonadotrophs, or on gonadotropin β-subunit mRNA expression in vitro (Gharib et al., 1990; Marshall et al., 1991). Based on these findings, it is very likely that gonadotrophs in the hamster pars tuberalis are not influenced by the direct (via estrogen receptors) and indirect (via hypothalamic GnRH) feedback actions of estradiol (Kawamoto et al., submitted). In addition, by employing using RT-PCR assay and in situ hybridization we found that exposure to a short-day photoperiod increased the incidence of gonadotropin β mRNA-expressing cells and the level of gonadotropin β mRNA in the hamster pars tuberalis, whereas these were decreased in the pars distalis. These photoperiod-mediated effects were blocked by pinealectomy (Kawamoto et al., 1999; Hozumi and Kawamoto, 2001). Expression of GnRH receptor mRNAs in the pars tuberalis and pars distalis also showed similar changes under different photoperiods. Although the possible role of the expression of gonadotropin genes increased by a short photoperiod remains unknown, the expression is up-regulated by melatonin. Conversely, previous investigators have reported that thyrotropin β gene expression in the hamster pars tuberalis is down-regulated after exposure to a short-day photoperiod (Wittkowski et al., 1999). In our study, the hormonal content of the pars tuber-alis was much lower than that of the pars distalis, as pointed out in rats (Chafuen and Cannata, 1979). These hormones in the pars tuberalis were immunologically identical to those in the pars distalis and show the same biological activity (Aguado et al., 1982). However, even if these hormones are released from the pars tubelalis, the amounts are too small to have much influence on target organs. More recent studies have revealed that the pars tuberalis secreted substances influencing the function of the pars distalis; unidentified tuberalin or 21 and 72kDa proteins (Guerra and Rodríguez, 2001; Stirland et al., 2001). Thus, the hypothesis that melatonin acts on cells in the pars tuberalis to regulate the production of an as yet unidentified substances may attract attention.

The presence of gonadotrophs in the bat pars tuberalis was first reported by Mikami et al. (1988a). In their report, LHβ -immunoreactive cells in the pars tuberalis were identified only in long-fingered bats by immunohistochemical analysis. In male rhinolophid bats, we found that LHβ-immunoreactive cells were distributed throughout the pars tuber-alis and their immunoreactivity varied among seasons (Fig. 3E–H). No other glycoprotein hormones were detected in the pars tuberalis of this species, although the use of antisera against other adenohypophysial hormones might identify the presence of other hormones in the pars distalis. LHβ immunoreactive cells in the pars tuberalis were encountered during early summer (recrudescent period) and mid-summer (spermatogenic period), but were not detected in autumn (mating period, Fig. 3G). These immunoreactive cells appeared again in winter (hibernation period, Fig. 3H). These observations show the seasonal variation in the secretory activity of LHβ in these cells, indicating that secretion of LHβ from the pars tuberalis is increased in autumn. Whether seasonal changes in LHβ immunoreactivity in the pars tuberalis are associated with gonadal activity, reproductive behavior or any specific environmental cues, such as photoperiod, remains to be elucidated.

Gonads

Gonadal steroids play various roles in the pars distalis, including cell proliferation and the regulation of hormone synthesis. Therefore, successful reproduction is critically dependent on the gonadal steroid feedback system. In bats, commonly measured steroid hormones are testosterone (T), P and estradiol (E) (Martin and Bernard, 2000), but no data are available for circulating glycoproteins of gonadal origin.

(1)Testicular function

Reproductive cyclicity in male bats inhabiting temperate latitudes can be classified into two patterns based on the timing and duration of testicular function (spermatogenesis in the testis and T production), the development of accessory sex organs and libido. Regardless of species, active spermatogenesis occurs during summer, which coincides with maximum development of the testes and the peak of circulating T levels.

First, asynchrony in activity between testes and accessory sex organs is noted in many species of bats. In these species, although the increase in testicular weight, spermatogenesis and the activity of Leydig cells occurs in summer, the development of accessory organs and mating behavior is retarded until autumn, by which time the testes have slightly or considerably regressed, the epididymides are full of spermatozoa, and the accessory sex organs show maximal development. Numerous spermatozoa stored within the epididymides remain after copulation throughout winter in both hibernating and nonhibernating species. Such asynchrony has been reported in vespertilinonid bats, M. lucifugus, Nyctalus noctula and Vespadelus vulturnus, and rhinolophid bats, R. ferrumequinum and R. capensis (Gustafson, 1979; Bernard, 1986; Martin and Bernard, 2000). This pattern of the male corresponds to the female pattern of delayed ovulation (Gustafson, 1979). In our study of R. ferrumequinum, after the arousal from hibernation, spermatogenesis started in mid-June and peaked in August, which is accompanied by the increase in testicular weight and circulating T levels (Fig. 6 A). In the mating period (mid-October), mature spermatozoa are full in the seminiferous tubules. Leydig cells are atrophic. Testicular weight and circulating T levels also decline in this season (Fig. 6A), and the accessory sex organs maximally develop in this period (Gustafson, 1979). For the peripheral endocrine mechanism on the asynchronous recrudescence between testis and the accessory sex organs, Gustafson and Damassa (1984, 1985) have pointed out that the increase in steroid binding globulin (SBG) produces high circulating T levels during spermatogenesis and inhibits the hypertrophy of accessory sex organs by reducing the free T available. Thus, fluctuations in SBG activity may be important factor in the recrudescence of the accessory sex organs.

Fig. 6

Seasonal changes in testes weight and serum T concentrations (A) and 125I-FSH-binding per unit tissue weight (B) and per testes (C) in adult bats. Other explanations are the same as for Fig. 2. Reprinted from Hayashi et al. (2002) with permission from Wiley-Liss, Inc.

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Second, male reproductive cyclicity is characterized by synchronized activity of the testes and accessory organs in several microchiropterian and many megachiropterian species. This pattern is generally the same as in other mammalian species, where all the male reproductive activities (the increase in spermatogenesis, testicular mass, circulating T levels and activity of the accessory sex organs) are synchronized. After copulation with a female, these activities decrease, resulting in a regression of testicular mass and accessory organs, and a decline in circulating androgen levels. This pattern has been reported in vespertilinonid bats, M. schreibersii (Gustafson, 1979; Bernard et al., 1991a) and pteropodid bats, Pteropus poliocephalus and P. scapulatus (McGuckin and Blackshaw, 1991a, b).

The primary hormonal control of spermatogenesis involves the actions of FSH and T on Sertoli cells, while LH initiates and maintains the steroidogenesis in Leydig cells. Several investigators have reported changes in gonadotropin receptors in the testis during the annual reproductive cycle in captive wild mammalian species (Calvo et al., 1986; Tsutsui et al., 1989; Howell-Skalla et al., 2000). To our knowledge, however, no data on gonadotropin receptors are available for bats and other mammalian hibernators. In rhinolophid bats, the concentrations of FSH receptors (specific binding per unit tissue weight) and total FSH receptors in the testes varied throughout the annual reproductive cycle (Fig. 6B, C). From scatchard plot analyses, these changes are considered due to variations in the number, not the affinity, of these receptors, as reported previously in other mammalian species (Abou-Isa and Reichert, 1977; Closset et al., 1977; Thanki and Steinberger, 1978; Barenton and Pelletier, 1983; Tsutsui et al., 1985; Calvo et al., 1986; Tsutsui et al., 1988). FSH receptors are primarily localized in Sertoli cells (Simoni et al., 1997) and therefore the decrease in the concentration of FSH binding sites on unit weight basis in summer (spermatogenic period) could be attributed to the dilution effect on Sertoli cells by germ cells. The elevated concentration of binding sites during the mating period is due to a rapid decrease of testicular germ cells, spermatocytes and spermatids, because there is some evidence that the absolute number of Sertoli cells per testis is stable after puberty and does not vary throughout the seasons in mammals (Steinberger and Steinberger, 1977; Sinha Hikim et al., 1988). From the histological examination of bat testis, the density of Sertoli cells per unit testicular weight appears to be considerably higher in the hibernating period than in other periods (Hayashi et al., 2002). Nevertheless, the concentration of binding sites and total binding sites in the testis, and FSH-stimulated production of cAMP (Fig. 7) were very low during hibernation. These results suggest that in the testis in the hibernating period, not only was the number of FSH binding sites per Sertoli cell extremely decreased, but the activity of the adenylate cyclase system led by the FSH-receptor complex was much reduced. LH and FSH secretion and the responsiveness of gonadotrophs to GnRH as assessed by cell immunoblot assay were markedly increased in the spermatogenic period compared to other periods (Fig. 5). Seasonal changes in FSH secretion without a secretagogue paralleled those in the total number of FSH binding sites. Thus, seasonal fluctuations are accompanied by corresponding changes in the responsiveness of testes to FSH, which appear to be due to the secretion of FSH from the pars distalis. Therefore, testicular FSH receptors might be up-regulated in response to elevated circulating FSH levels in bats. After the arousal from hibernation elevated FSH secretion may be required for the initiation of testicular recrudescence and expression of FSH receptors, which seems to be supported by an elevated FSH secretion, and the testicular weight is concomitantly increased during testicular recrudescent in hamsters (Berkowitz and Heindel, 1984; Milette et al., 1988; Schatt et al., 1995).

Fig. 7

Dose effect of FSH stimulation on cAMP accumulation in cultured seminiferous tubules in adult bats. Tissue was incubated with shaking for 60 min at 35°C in an atmosphere of 95% air and 5% CO2. Other explanations are the same as for Fig. 2. Reprinted from Hayashi et al. (2002) with permission from Wiley-Liss, Inc.

i0289-0003-20-9-1057-f07.gif

(2) Ovarian Function

As mentioned above, female bats inhabiting temperate latitudes show unusual reproductive phenomena, a delayed ovulation/fertilization or a delayed implantation that is temporarily interrupted by hibernation during winter (Oxberry, 1979). In vespertilionid and rhinolophid bats showing the former, estrus and copulation occur in late summer or autumn and spermatozoa are stored during winter in the female reproductive tract. In Antrozonus pallidus, the ovaries contain a developed Graffian follicle and circulating P levels are relatively low throughout the period, whereas E levels increase temporarily at estrus (Oxberry, 1979). Such low P levels seem to be prerequisite for the maintenance of delayed ovulation. After arousal from hibernation, circulating E levels appear to rise temporarily at ovulation and then decline immediately after ovulation. As the pregnancy proceeds, E levels dramatically increase reaching a peak at mid-pregnancy and a subsequent increase occurs during lactation. Circulating P levels also increase after the arousal, particularly with the development of the corpus luteum after ovulation. In this period, 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase, enzymes necessary for converting to P and E respectively, are strongly expressed in the interstitial tissue and corpus luteum (Oxberry, 1979). At the early stage of pregnancy, P levels temporarily decline and then increase considerably to reach a peak prior to parturition. Changes of these steroidal levels are similar in several other species showing a delayed ovulation/fertilization pattern, such as M. lucifugus (Buchanan and Younglai, 1986, 1988) and Chalinolobus gouldii (Hosken et al., 1996).

As to the delayed implantation pattern, several reports are present in cosmopolitan species, M. schreibersii. In general, circulating P levels are quite low during delayed implantation and not significantly different from those of non-pregnant females. Significantly elevated P levels were observed during fetal development accompanied by development of the placenta, reaching peaks for one month prior to parturition (Martin and Bernard, 2000). A comparison of data from different populations of M. schreibersii in the world may be useful (Martin and Bernard, 2000).

CONCLUSIONS AND PERSPECTIVES

Bats are highly successful mammals, second to rodents in the number of individuals and the diversity of species. Their reproductive pattern, the timing and duration also vary considerably among species and different habitats. The annual changes in the hypothalamo-adenohypophysialgonadal axis and other endocrine characteristics in bats have been reviewed here, based mainly on our studies in hibernating rhinolophid bats, R. ferrumequinum. General summary is illustrated in Fig. 8. Although knowledge has considerably been accumulated on reproductive biology in chiropterian species, precise neuroendocrine mechanism regulating the reproduction of bats remains unsolved.

Fig. 8

Summarized endocrine characteristics and control in bats living in the temperate zone. A: female bats showing delayed implantation pattern, B: female bats showing delayed ovulation/fertilization pattern, C: male bats showing asynchronized recrudescence. ASO: accessory sex organs, Gn: gonadotropin, Gn-R: gonadotropin receptor, TW: testicular weight. : increase, : decrease, +: acceleration or stimulation, – : inhibition. Other abbreviations are cited in the text.

i0289-0003-20-9-1057-f08.gif

The reproductive activities in wild mammalian species are influenced by environmental factors, such as photoperiod, ambient temperature, relative humidity, food availability, and so on. In bats inhabiting temperate zones, the period of torpor induced by low ambient temperature and food deprivation act to arrest or retard the progress of reproductive events (Racey, 1982). In the natural environment of the temperate zones, annual changes in photoperiod are accompanied by simultaneous changes in ambient temperature. The available food becomes poor before winter. In bats, spermatogenesis occurs in summer, the season with a long-day photoperiod, but mating takes place when daylengths become short and gonadal activity is decreased (autumn). In general, seasonal breeding mammals are classified as long-day or short-day breeders according to which day-length stimulates reproductive activity. However, nocturnal bats are difficult to categorize on this basis owing to a seasonally separated asynchrony associated with reproductive phenomena. Beasley et al. (1984) and Beasley and Zucker (1984) showed in microchiropteran bats, A. pallidus (Vespertilionidae), that exposure to a short-day photoperiod or melatonin treatment accelerated testicular regression to the autumn level, suggesting that these treatments influenced the reproductive functions by affecting the endogenous circannual reproductive rhythm. In megachiropteran bats, P. poliocephalus (Pteropodidae), however, several investigators showed that photoperiod is unlikely to be a major environmental factor affecting reproductive activity (McGuckin and Blackshaw, 1992; O'Brien et al., 1993). Clarification of whether such contradictory results are due to species variation or the difference in habitat latitude will require further studies.

In the past decade, major advances in endocrinology have been made in determining the chemical structure of hormones and receptor genes and examining the regulation of gene expression. Despite continuing efforts, studies at the molecular level and the establishment of a homologous assay for bat adenohypophysial hormones have not been done. The lack of such investigations seems to be in part due to difficulty in rearing bats in the laboratory. According to results by other laboratories, long-term feeding in the laboratory is possible in some species of vespertilionid bats. In our preliminary trial, feeding was successful for a few species of vespertilionid bats for more than a year. Unfortunately, however, reproduction has not been succeessful in the laboratory except for megachiropteran bats. The lack of suitable experimental model in bats seems to be the greatest problem to be solved.

Acknowledgments

The author thanks Dr. Seiichiro Kawashima, Emeritus Professor of University of Tokyo and Director of Research Laboratory, Zenyaku Kogyo Co., Ltd. for his critical reading of the manuscript and helpful suggestions.

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Keiichi Kawamoto "Endocrine Control of the Reproductive Activity in Hibernating Bats," Zoological Science 20(9), 1057-1069, (1 September 2003). https://doi.org/10.2108/zsj.20.1057
Accepted: 1 June 2003; Published: 1 September 2003
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