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1 August 2010 Biochemical and Immunohistochemical Analyses of a GnRH-like Peptide in the Neural Ganglia of the Pacific Abalone Haliotis Discus Hannai (Gastropoda)
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Abstract

We examined whether gonadotropin-releasing hormone (GnRH)-like peptides are present in the neural ganglia of the gastropod Pacific abalone (Haliotis discus hannai) by reverse-phase high performance liquid chromatography (rpHPLC) combined with time-resolved fluoroimmunoassay (TR-FIA) analysis and by immunohistochemistry. Cerebral ganglion extracts showed a similar retention time to lamprey GnRH-II (lGnRH-II) in rpHPLC combined with TR-FIA analysis. GnRH-like-immunoreactive (ir) cell bodies (which reacted with a mouse monoclonal antibody raised against the common amino acid sequence of vertebrate GnRH) were detected in the peripheral region of the cerebral ganglion, and they were observed to send fibers into the neuropil. GnRH-like-ir fibers were also detected in the neuropil of the pedal ganglion, the visceral nerve, and the nerve originating from the pedal ganglion. Chicken GnRH-II (cGnRH-II)-like-ir fibers (which reacted with a rabbit polyclonal antibody raised against cGnRH-II) were also observed in the neuropil of the cerebral ganglion. GnRH-like-ir fibers and cGnRH-II-like-ir fibers were distinguishable in the neuropil of the cerebral ganglion by double-staining immunohistochemistry. These results suggest that multiple GnRH-like peptides exist in the neural ganglia of the Pacific abalone.

© 2010 Zoological Society of Japan
Masafumi Amano, Shunsuke Moriyama, Kataaki Okubo, Noriko Amiya, Akiyoshi Takahashi, and Yoshitaka Oka "Biochemical and Immunohistochemical Analyses of a GnRH-like Peptide in the Neural Ganglia of the Pacific Abalone Haliotis Discus Hannai (Gastropoda)," Zoological Science 27(8), 656-661, (1 August 2010). https://doi.org/10.2108/zsj.27.656
Received: 16 December 2009; Accepted: 1 March 2010; Published: 1 August 2010
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