Numerous isolated teeth, fin spine fragments and dermal denticles of a hybodont shark from a lacustrine limestone horizon at the top of lithofacies B of the Late Carboniferous to Early Permian succession of the Guardia Pisano Basin (Sulcis area, southwestern Sardinia, Italy) are assigned to a new species of the genus Lissodus Brough, 1935. Lissodus sardiniensis sp. nov. is erected on the basis of about 500 teeth, which show a unique feature of only one pair of lateral cusps that are bent in the direction of the prominent central cusp. Weak heterodonty allows distinction of symphyseal, mesial to anterolateral, and lateral teeth. Lissodus sardiniensis sp. nov. was a freshwater-adapted durophagous shark of bottom dwelling habit, an interpretation supported by general construction of the dentition and the morphology of the dermal denticles. The association with Acanthodes, diplodoselachid sharks and branchiosaurs allows the reconstruction of a five-level trophic chain for the Guardia Pisano Basin. The discovery of Lissodus in Sardinia is presently the southernmost known occurrence of that genus in the Late Palaeozoic of Europe. This new find adds significantly to knowledge of migration routes of aquatic organisms, especially freshwater sharks, between the single European basins in the Late Pennsylvanian, and changes in palaeobiogeography during the Early Permian.