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23 March 2021 Effect of the Bacterium Paenibacillus larvae larvae on Vitellogenin Gene Expression of the Queen Honey Bee Apis mellifera L.
S.A.S. Gomaa, E.M.S. Barakat, M.S. Salama, E.E. El-Gohary
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Abstract

Virgin queens of the honey bee Apis mellifera L. were assessed following feeding in fourth larval instar with a sublethal dose of bacterium Paenibacillus larvae larvae (LD20 = 1.07 × 102 colony forming units (CFU)/queen), the causative agent of American foulbrood disease (AFB). The extraction of ovary proteins showed a highly significant increase for treated queens. The bacterium P. l. larvae was detected by conventional PCR-technique within treated queens ovaries with molecular size of 550 bp. Vitellogenin gene has been expressed in healthy and treated queens using RT-PCR technique, up-regulation took place in treated queens, vitellogenin was found with a high level of gene expression (∼20-fold). Finally, this study could contribute to initial efforts to help attempts to breed honey bee queens that are resistant to the pathogen.

©Entomological Society of Southern Africa
S.A.S. Gomaa, E.M.S. Barakat, M.S. Salama, and E.E. El-Gohary "Effect of the Bacterium Paenibacillus larvae larvae on Vitellogenin Gene Expression of the Queen Honey Bee Apis mellifera L.," African Entomology 29(1), 96-103, (23 March 2021). https://doi.org/10.4001/003.029.0096
Received: 20 November 2019; Accepted: 16 November 2020; Published: 23 March 2021
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