The mygalomorph spider genus Myrmekiaphila comprises 11 species known only from the southeastern United States. The type species, M. foliata Atkinson, is removed from the synonymy of M. fluviatilis (Hentz) and placed as a senior synonym of M. atkinsoni Simon. A neotype is designated for M. fluviatilis and males of the species are described for the first time. Aptostichus flavipes Petrunkevitch is transferred to Myrmekiaphila. Six new species are described: M. coreyi and M. minuta from Florida, M. neilyoungi from Alabama, M. jenkinsi from Tennessee and Kentucky, and M. millerae and M. howelli from Mississippi.