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Herein are described and illustrated the mature larvae of the following species assigned to the Euglossini: Eufriesea surinamensis (Linnaeus), Eufriesea mussitans (Fabricius), Euglossa (Euglossa) cordata (Linnaeus), Eulaema (Apeulaema) polychroma (Mocsáry). They are compared both with one another and with previous accounts of other taxa assigned to the tribe, and a taxonomic key is presented. The paper concludes with a preliminary larval characterization of the tribe.