Ophiopogon yangshuoensis R.H. Jiang & W.B. Xu (Asparagaceae), a new species from limestone areas in Guangxi, China, is described and illustrated. It is similar to O. intermedius, but differs by its abaxially glaucous leaves, white streaked, broadlanceolate or ovate-lanceolate bracts, narrow-lanceolate innermost bracts, and ovate, reflexed tepals.