Pleurothallopsis renziana sp. nova (Orchidaceae) is described based on Venezuelan material. It resembles P. microptera in which, however, the sepals are longer, the dorsal sepal is oblong-ovate, somewhat constricted in apical third, the lip is widest in the apical third, with small lateral lobes, and ornamented exclusively with one pair of low calli. The flower morphology of P. renziana resembles that of P. tubulosa, but the former differs from the latter by the larger plant size and longer leaves, obliquely linear-ovate lateral sepals, petals being widest in the basal third or in the middle, lip being distinctly wider across lateral lobes than across middle lobe, convex basal part of lip, presence of a pair of low calli extending from the lip lateral lobes' base, and an additional keel running along the central part of the lip.