Antennoseius (Vitzthumia) ventrianalis sp. nov. is the second species of Antennoseius described from Australia (Acari: Ascidae). It is described from non-phoretic specimens collected from the soil surface. The new species is unique in having three pairs of ventral setae on the ventri-anal shield; the maximum observed in other species is two pairs. It is sexually dimorphic in the degree of fusion of the podonotal and opisthonotal shields and some other minor characters.