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1 September 2007 Phylogenetic Analyses and a Method for Rapid Molecular Diagnosis of Two Sunflower Seed Weevils (Coleoptera: Curculionidae)
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Abstract

The red sunflower seed weevil, Smicronyx fulvus LeConte, and gray sunflower seed weevil, Smicronyx sordidus LeConte (Coleoptera: Curculionidae), are among the most important seed pests of cultivated sunflowers, Helianthus annuus L., in the northern Great Plains. A portion of 18S ribosomal DNA (18S rDNA) sequence from specimens of 31 populations of the morphologically similar species that were collected across a wide geographic region of the United States showed a consistent separation of the two species. The restriction endonuclease HaeII digests the piece of 18S rDNA in only one of the two species, and this restriction fragment-length polymorphism can be used for species diagnosis. Phylogenetic analysis for species of four tribes of the weevil subfamily Erirhininae showed that the sunflower seed weevil tribe, Smicronychini, is monophyletic.

Shujuan Li, Lu Sun, Christian Y. Oseto, and Virginia R. Ferris "Phylogenetic Analyses and a Method for Rapid Molecular Diagnosis of Two Sunflower Seed Weevils (Coleoptera: Curculionidae)," Annals of the Entomological Society of America 100(5), 649-654, (1 September 2007). https://doi.org/10.1603/0013-8746(2007)100[649:PAAAMF]2.0.CO;2
Received: 22 September 2006; Accepted: 1 May 2007; Published: 1 September 2007
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