Translator Disclaimer
1 March 2013 Cloning and Characterization of the Caspase Family Member BmDronc from Bombyx mori (Lepidoptera: Bombycidae) Embryo Cells
Jin-Ye Zhang, Wei Xu, Chun Pan, Hua-Shan Yi, Yan-Fen Hu, Juan Song, Min-Hui Pan, Cheng Lu
Author Affiliations +
Abstract

Caspases are a family of cysteinyl aspartate proteinases known for their essential roles in the regulation of apoptosis. To date, ≈20 caspases have been identified, although few have been cloned and characterized in Bombyx mori (L.). Two caspase-9 variants from the B. mori embryo cell line (BmE), designated BmDroncL and BmDroncS, were cloned. BmDroncL has three classic domains: a caspase recruitment domain and large and small subunits, and is a homologue of Drosophila Dronc, human caspase-9, Aedes Dronc, and fish caspase-9, whereas BmDroncS lacks the large subunit. Instantaneous expression of BmDroncL caused typical apoptosis in Sf-9 cells. Moreover, BmDronc gene expression increased markedly during the silkworm metamorphosis at the larva-pupa and pupa-moth stages, which suggested that BmDronc plays a crucial role during B. mori metamorphosis.

© 2013 Entomological Society of America
Jin-Ye Zhang, Wei Xu, Chun Pan, Hua-Shan Yi, Yan-Fen Hu, Juan Song, Min-Hui Pan, and Cheng Lu "Cloning and Characterization of the Caspase Family Member BmDronc from Bombyx mori (Lepidoptera: Bombycidae) Embryo Cells," Annals of the Entomological Society of America 106(2), 265-272, (1 March 2013). https://doi.org/10.1603/AN12110
Received: 4 September 2012; Accepted: 1 January 2013; Published: 1 March 2013
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top