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1 July 2015 Subcellular Localization, Transcription, Expression, and Activity of Dihydrolipoamide Dehydrogenase (DLDH) during Developmental Stages and in Different Tissues of Silkworm (Lepidoptera: Bombycidae)
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Abstract

Dihydrolipoamide dehydrogenase (DLDH) is a multifunctional oxidoreductase and is well known as an essential component of four mammalian mitochondrial multienzyme complexes: pyruvatedehydrogenase, α-ketoglutarate dehydrogenase, branched-chain α-keto acid dehydrogenase, and the glycine cleavage system. In the previous study, we described the cloning, purification, and oxidoreductase activity of recombinant silkworm, Bombyx mori DLDH (BmDLDH) expressed in Escherichia coli. In the present work, the result showed that 1) BmDLDH was present in both mitochondria and cytoplasm, but BmDLDH in mitochondria was more abundant than that in cytoplasm, which indicated the BmDLDH could be dual localized. 2) Both the expression levels and diaphorase activity of BmDLDH in silkworm first instars and moth were higher than those in other development stages. The transcript levels of BmDLDH were not different in developmental stages. 3) The transcription, expression, and activity of BmDLDH in Malpighian tubule were higher than those in other tissues.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Yang Zhou, Juan Huo, Ting-Ya Jiang, Heng-Chuan Xia, Hai-Tao Liu, Xiao Yuan, Rong-Fa Xu, Ke-Ping Chen, and Hai-Feng Shi "Subcellular Localization, Transcription, Expression, and Activity of Dihydrolipoamide Dehydrogenase (DLDH) during Developmental Stages and in Different Tissues of Silkworm (Lepidoptera: Bombycidae)," Annals of the Entomological Society of America 108(4), 562-569, (1 July 2015). https://doi.org/10.1093/aesa/sav021
Received: 2 November 2014; Accepted: 3 March 2015; Published: 1 July 2015
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