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1 July 2015 Identification and Characterization of a Novel Methionine Sulfoxide Reductase B Gene (AccMsrB) from Apis cerana cerana (Hymenoptera: Apidae)
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Abstract

Methionine sulfoxide reductase B genes (MsrBs) play a crucial role protecting cells from oxidative damage. In this study, we isolated and characterized an MsrB gene from Apis cerana cerana F., designated AccMsrB. The full cDNA of AccMsrB was 757 bp with an open reading frame (ORF) of 414 bp, and the predicted translation product was a 137 amino acid polypeptide with an estimated molecular mass of 15.5 kDa and an isoelectric point of 7.77. Multiple sequence alignment revealed that AccMsrB shares high identity with other known MsrBs (Apis florea F., Apis mellifera L., Apis dorsata F., and Bombus terrestris L.) and contains conservative Cys residues. Quantitative real-time polymerase chain reaction showed AccMsrB to be highly expressed in the epidermis of adult workers, reaching high levels in first-instar larvae, prepupae, and 15-d-old adults. Furthermore, the expression of AccMsrB was upregulated by various oxidative stresses, including 4, 16, 25, and 42°C, ultraviolet light (30mj/cm2), H2O2, CdCl2, HgCl2, paraquat, imidacloprid, and cyhalothrin. However, AccMsrB was downregulated when exposed to phoxime. These results indicate that AccMsrB might respond to various environmental stresses and protect against reactive oxygen species.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Feng Liu, Zhihong Gong, Weixing Zhang, Ying Wang, Lanting Ma, Hongfang Wang, Xingqi Guo, and Baohua Xu "Identification and Characterization of a Novel Methionine Sulfoxide Reductase B Gene (AccMsrB) from Apis cerana cerana (Hymenoptera: Apidae)," Annals of the Entomological Society of America 108(4), 575-584, (1 July 2015). https://doi.org/10.1093/aesa/sav042
Received: 12 February 2015; Accepted: 17 April 2015; Published: 1 July 2015
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