In the Monte Pisano area (Tuscany, central Italy), spiders were collected within two research projects during three years (2010, 2013, 2014). Olive groves and adjacent semi-natural habitats (wood and Mediterranean garrigue) were investigated with three sampling methods (pitfall trapping, beating at branches and hand collection in the canopy). A total of 148 species was identified. The ground spider (Gnaphosidae) Zelotes fulvaster (Simon, 1878) was recorded for the first time in Italy.