The present work offers records of six species of spiders (Arachnida: Araneae) from three localities in the eastern part of the Republic of the Sudan. Three represent new species records for the country. Artema kochi (Kulczyński, 1901) (Pholcidae) was collected in New Halfa in Kassala State. Pardosa observans (O. Pickard-Cambridge, 1876) (Lycosidae) was found in the Dinder National Park in Sennar State, while Pterotricha dalmasi (Fage, 1929) (Gnaphosidae) was recorded from Toker and Khalig Dongnab in Red Sea State. Additionally, the previously recorded species, Eusparassus walckenaeri (Audouin, 1826) (Sparassidae) is recorded for the first time from the Dinder National Park in Sennar state, and Langona alfensis (Heciak & Prószyński, 1983) and Menemerus fagei (Berland & Millot, 1941) (both Salticidae) are reported for the first time from New Halfa in Kassala State.