Two new species of the genus Chapoda Peckham & Peckham, 1896 are described and illustrated from southern Ecuador. Both new species C. guancavilca n. sp. (♂♀) and C. santay n. sp. (♀) are described and illustrated from Puná and Santay Islands, Guayas province. In addition, two new genera records are reported from Ecuador: Bellota Peckham & Peckham, 1892 (with B. formicina (Taczanowski, 1878)) and Martella Peckham & Peckham, 1892 (with M. gandu Galiano, 1996), along with a new species record of Lyssomanes elegans (F. O. Pickard-Cambridge, 1900). Finally, new faunistic records are given for Corythalia scutellaris (Bayer, 2020), Frigga crocuta (Taczanowski, 1878), F. opulenta (Galiano, 1979), and L. unicolor (Taczanowski, 1871). A distribution map with these and previously published records on the genera here analysed is provided.