Aquatic macrophytes provide foraging and refuge habitats for a wide variety of macroinvertebrates, including spiders. Examination of Salvinia auriculata plants in lagoons in southeastern Brazil resulted in 44 records of spiders belonging to seven families: Cheiracanthiidae, Linyphiidae, Lycosidae, Pisauridae, Salticidae, Tetragnathidae, and Theridiidae. At least seven morphospecies were identified. It is noteworthy that 61.3% of the sampled individuals were young spiders.