Some cormorant species are perceived as negatively interacting with fish resources but conclusive evidence supporting such suggestions are scarce. Here we studied the diet of the neotropic cormorant and assessed its impact on recreational fish resources. A total of 33 pellets (regurgitated casts) and 73 regurgitations produced by the Neotropic cormorant were collected between 19 October 2006 and 24 February 2007 at a colony on the Carrileufu River, Patagonia, Argentina. Fish (94.3%) were the most abundant prey, followed by crustaceans (5.1%) and molluscs (0.6%). The Patagonian silverside Odontesthes hatcheri was the most frequent fish prey, followed by the rainbow trout Oncorhynchus mykiss and the creole perch Percichthys trucha. The contribution of fish species to the diet varied throughout the breeding season. The fish intake estimate for the colony based on parameters drawn from previous studies was 4.708 tons, which represents a consumption of 41,504 fish specimens. Native species were the major component of the cormorant diet, comprising 75.7% of the specimens and 73.2% of the biomass consumed during the course of the breeding season. Given fish availability and productivity within the study area, the impact on recreational fish resources by Neotropic cormorants can be estimated to be of minor importance.