Translator Disclaimer
1 October 2002 Comparison of a Putative Second Serotype of Chicken Infectious Anemia Virus with a Prototypical Isolate II. Antigenic and Physicochemical Characteristics
Author Affiliations +
Abstract

A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated included thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenically by enzyme-linked immunosorbent assay, virus neutralization, immunofluorescence assay, and western blot. All four serologic assays demonstrated that CIAV-7 is antigenically distinct from the Del-Ros strain of CIAV. Additionally, polymerase chain reaction (PCR) and Southern blot were used to determine if there were similarities in genome sequence between the two viruses. CIAV-7 could not be detected with CIAV-specific PCR primers or a with CIAV-specific probe by Southern hybridization.

E. Spackman, S. S. Cloud, and J. K. Rosenberger "Comparison of a Putative Second Serotype of Chicken Infectious Anemia Virus with a Prototypical Isolate II. Antigenic and Physicochemical Characteristics," Avian Diseases 46(4), 956-963, (1 October 2002). https://doi.org/10.1637/0005-2086(2002)046[0956:COAPSS]2.0.CO;2
Received: 9 April 2002; Published: 1 October 2002
JOURNAL ARTICLE
8 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top